聚乙二醇_PEG6000_模拟干旱胁迫抑制矮沙冬青种子的萌发_于军.pdfVIP

基因组学与应用生物学，2010 年，第29 卷，第2 期，第355-360 页 Genomics and Applied Biology, 2010, Vol.29, No.2, 355-360 研究报告 A Letter 聚乙二醇(PEG6000)模拟干旱胁迫抑制矮沙冬青种子的萌发 1 2* 于军 焦培培 1 塔里木大学科技处, 阿拉尔, 843300; 2 新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室, 阿拉尔, 843300 * 通讯作者, jiaopeipei2000@ 摘 要 本研究以新疆特有濒危保护植物矮沙冬青(Ammmopiptanthus nanus (M. Pop.) Cheng f.) 的种子为材 料，用不同渗透势浓度的聚乙二醇(PEG6000)模拟干旱胁迫，探讨干旱胁迫对矮沙冬青种子发芽率、平均发芽 速度、胚轴和胚根长度及发芽指数、活力指数的影响。结果表明，不同浓度PEG 胁迫处理均降低了种子的发芽 率，延缓了矮沙冬青种子萌发进程；种子的发芽率、发芽指数和活力指数均随胁迫强度的增加呈明显下降趋 势。当-1.20 MPa 的PEG 胁迫处理的种子在试验结束时仍未能萌发，表明-1.20 MPa 是矮沙冬青种子萌发的 临界水势。PEG 模拟干旱胁迫中，当PEG 处理为-0.2 MPa 时，虽然最终发芽率与对照一样，但其胚根、胚轴的 长度都比对照短，说明矮沙冬青胚根、胚轴的生长比发芽率对干旱胁迫更敏感。干旱胁迫可能是导致矮沙冬青 种群天然更新能力弱的原因之一。本研究将为矮沙冬青种质资源的保护和种群的恢复提供科学依据。 关键词 矮沙冬青, 聚乙二醇(PEG6000), 干旱胁迫, 种子萌发 Inhibition of Seed Germination of Ammmopiptanthus nanus (M. Pop.) Cheng f. under Simulated Drought Stress with Polyethylene Glycol (PEG6000) Yu Jun 1 Jiao Peipei 2* 1 Science and technology department of Tarim Univeisity, Alar, 843300;2 Key Laboratory of Biological Resource Protection and Utilization of Tarim Basin, Xinjiang Production Construction Group, Alar, 843300 * Corresponding author, jiaopeipei2000@ DOI: 10.3969/gab.029.000355 Abstract In this paper, we have taken the seeds of endangered species Ammmopiptanthus nanus (M. Pop.) Cheng f. as the test material, and studied on the effects of simulated drought stress with different concentration and osmotic potential of polyethylene glycol (PEG6000) on their seminal germination, such as germination capacity, average speed of germination, length of radicle and hypocotyls, germination index and viability index. The results showed that PEG could retard the proceeding of seeds germination of Ammmopiptanthus nanus (M.Pop.) Cheng f. and the germination percentage, germination index, and seed viability index were all showed the downtrend obviously with increasing concentration of PEG. The seeds treated with -1.20 MPa water potential of PEG could not germinate at all