收稿日期2015-05-13.doc

收稿日期：2015-05-13 基金项目：江西省卫生和计划生育委员会2014A008）（ZX1013）大学生创新创业基金资助课题。 作者简介：韦国兵（1976-），男，湖南沅江人，副教授，硕士，硕士生导师，研究方向：药物质量标准与中药活性成分分析，Email:weigb@163.com。 吴茱萸中吴茱萸碱和吴茱萸次碱含量的HPLC测定 韦国兵，毛玲，何玲玲，张秀秀 （江西中医药大学 药学院，江西 南昌 330004） 摘要：目的：采用高效液相色谱法建立一种新的同时测定吴茱萸中吴茱萸碱和吴茱萸次碱含量的方法，为吴茱萸的质量控制提供依据。方法：采用Kromasil 100-5C18ODS2色谱柱(5μm,250mm×4.6mm)，(51：49)1.0mL/min，290nm。0.1～1.0μg范围内与峰面积呈良好线性关系，吴茱萸碱回归方程是Y=1130.7X+2.9721，r2=0.9998(n=6)100.26%，RSD为1.87%；Y=916.84X-9.9512，r2=0.9994(n=6)100.37%，RSD为2.64%。R944.5 文献标识码：A 文章编号：1671-380X(2015)09-0000-03 Determination of the Contents of Evodiamine and Rutaecarpine in FRUCTUS EVODIAE by HPLC WEI Guo-bing, MAO Ling, HE Ling-ling, et al (College of Pharmacy, Jiangxi University of Chinese Medicine, Nanchang 330004, China) ABSTRACT：Objective: The aim is to determine a new content of evodiamine and rutaecarpine in FRUCTUS EVODIAE with HPLC and provide the basis for the quality control of FRUCTUS EVODIAE amount. Methods: The chromatographic separation was performed on Kromasil 100-5C18ODS2 column (5μm,250mm×4.6mm) with mobile phase of acetonitrlie-water(51:49) and flow rate was 1.0mL/min. The samples were detected at the wavelength of 290 nm. Results: The amount of evodiamine and rutaecarpine was respectively at the ranges of 0.1～1.0μg, which showed a good linear correlation between the ranges and the peak area. The equation of linear regression of evodiamine was Y=1130.7X+2.9721,r2=0.9998(n=6), the recovery rate of evodiamine was 100.26% and RSD was 1.87%. The equation of linear regression of rutaecarpine was Y=916.84X-9.9512, r2=0.9994(n=6), the recovery rate of rutaecarpine was 100.37% and RSD was 2.64%. Conclusions: This method is easy, fast, accurate and reliable and may provide the basis for the quality control of FRUCTUS EVODIAE. Key words：Evodiamine; Rutaecarpine; HPLC; Determination of Content 吴茱萸（FRUCTUS EVODIAE）Evodia rutaecarpa (Juss.) Benth、石虎Evodia rutaecarpa (Juss.) Benth. var. officinalis (Dode)Huang或疏毛吴茱萸Evodia rutaecarpa (Juss.