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QIAGEN 试剂盒
QIAamp? DNA FFPE Tissue
Handbook
For purification of genomic DNA from
formalin-fixed, paraffin-embedded tissues
April 2010
Sample Assay Technologies
1063027-HB 28.04.2010 10:33 Uhr Seite 1
Trademarks: QIAGEN?, QIAamp?, MinElute?, Qproteome?, REPLI-g?, RNeasy? (QIAGEN Group).
? 2007 QIAGEN, all rights reserved.
QIAGEN is a member of the Forest Stewardship Council (FSC). For the production of printed materials,
including handbooks, QIAGEN has a policy to select suppliers that comply with FSC standards for printing
processes and well-managed forests.
1063027-HB 28.04.2010 10:33 Uhr Seite 2
QIAamp DNA FFPE Tissue Handbook 04/2010 3
Contents
Kit Contents 4
Storage 4
Quality Control 4
Product Warranty and Satisfaction Guarantee 5
Technical Assistance 5
Safety Information 6
Product Use Limitations 7
Introduction 8
Principle and procedure 8
Equipment and Reagents to Be Supplied by User 10
Important Notes 11
Starting material 11
Copurification of RNA 11
Eluting pure DNA 11
Handling of QIAamp MinElute columns 12
Preparation of buffers 13
Protocol
■ Isolation of Genomic DNA from FFPE Tissue Sections 14
Troubleshooting Guide 17
Appendix: Working with DNA 19
Ordering Information 20
1063027-HB 28.04.2010 10:33 Uhr Seite 3
Kit Contents
QIAamp DNA FFPE Tissue Kit (50)
Catalog no. 56404
Number of preps 50
QIAamp MinElute? Columns 50
Collection Tubes (2 ml) 3 x 50
Buffer ATL 10 ml
Buffer AL* 12 ml
Buffer AW1* (concentrate) 19 ml
Buffer AW2? (concentrate) 13 ml
Buffer ATE? 12 ml
Proteinase K 1.25 ml
Handbook 1
* Contains a guanidine salt. Not compatible with disinfectants containing bleach. See page 6 for safety
information.
? Contains sodium azide as a preservative.
Storage
QIAamp MinElute columns should be stored at 2–8°C upon arrival and are stable under
these conditions for at least one year after delivery. However, short-term storage of up
to 4 weeks at room temperature (15–25°C) does not affect performance.
All buffers can be stored at room temperature (15–25°C) an
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