- 1、本文档共6页,可阅读全部内容。
- 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
- 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载。
- 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
查看更多
qPCR absolute quantification
Journal of Immunological Methods 354 (2010) 34–39
Contents lists available at ScienceDirect
Journal of Immunological Methods
j ourna l homepage: www.e lsev ie / locate / j imResearch paper
Comparison of different standards for real-time PCR-based
absolute quantification
S. Dhanasekaran a,b, T. Mark Doherty c,d, John Kenneth a,?
and TB Trials Study Group
a Infectious Diseases Unit, St. Johns Research Institute, Koramangala, Bangalore – 560 034, India
b The Gade Institute, Section for Microbiology and Immunology, University of Bergen and Haukeland University hospital, N-5021, Norway
c Infectious Disease Immunology, Statens Serum Institute, Artillerivej 5, Copenhagen 2300s, Denmark
d Center for International Health, University of Bergen, N-5021, Norwaya r t i c l e i n f oAbbreviations: qPCR, Quantitative real-time PCR;
R2, Correlation coefficient; HuPO, Human acidic ribos
IFN γ, Interferon gamma.
? Corresponding author. Tel.: +91 80 fa
E-mail address: johnkennt@ (J. Kenneth
0022-1759/$ – see front matter ? 2010 Elsevier B.V.
doi:10.1016/j.jim.2010.01.004a b s t r a c tArticle history:
Received 3 October 2009
Received in revised form 8 January 2010
Accepted 8 January 2010
Available online 25 January 2010Quantitative real-time PCR (qPCR) is a powerful tool used for both research and diagnostic,
which has the advantage, compared to relative quantification, of providing an absolute copy
number for a particular target. However, reliable standards are essential for qPCR. In this study,
we have compared four types of commonly-used standards — PCR products (with and without
purification) and cloned target sequences (circular and linear plasmid) for their stability during
storage (using percentage of variance in copy numbers, PCR efficiency and regression curve
correlation coefficient (R2)) using hydrolysis probe (TaqMan) chemistry. Results, expressed as
copy numbers/μl, are presented from a sample human system in which absolute levels of HuPO
(reference gene) and th
您可能关注的文档
- On the Group of Automorphisms of Universal Algebra and Many Sorted Algebra.pdf
- On the long term spatial segregation for a competition-diffusion system.pdf
- On the number of bound states for Schrdinger operators with operator-valued potentials.pdf
- On the mechanisms of various fretting wear modes微动磨损.pdf
- On the operator space UMD property for noncommutative Lp-spaces.pdf
- On the Polyharmonic Operator with a Periodic Potential.pdf
- On the position operator for massless particles.pdf
- On the semi-regular module and vertex operator algebras.pdf
- On the Trivial Many Sorted Algebras and Many Sorted Congruences.pdf
- On the union stabilization of two Heegaard splittings.pdf
文档评论(0)