宫颈永生化细胞和癌细胞胞膜差异蛋白质组学研究.pdfVIP

. 1336. 四川医学 2011 年 9 月第 32 卷(第 9 期) Sichuan Medical 10umal ,2011 , Vol. 32 ,No.9 宫颈永生化细胞和癌细胞胞膜差异蛋白质组学研究 宋大萍 1 ，赵涌 2 (1.绵阳市第三人民医院病理科，四川绵阳 621∞0;2. 重庆医科大学病理教研室，重庆 4∞016) [摘要] 目的 建立 HPV-16 阳性的宫颈上皮永生化细胞(H8 细胞株)和宫颈癌细胞(CaSKi 细胞株)胞膜蛋白双向 电泳图谱，寻找特征性的差异蛋白点。方法提取两种细胞胞膜蛋白质进行双向凝胶电泳(2-dimensronal gel electrophore- sis , 2-DE) ，建立电泳图谱，然后筛选出差异点进行基质辅助激光解吸飞行时间串联质谱( matrix-assisted laser desorption/i- onization time-of-flight tandem mass spectrome町， MAWI-TOF-MS/MS) 分析，鉴定出蛋白质。结果 获得了分辨率和重复性 均较好胞膜蛋白 2-DE 图谱，并分析找出了 13 个的差异蛋白点，对其中 3 个显著差异点进行质谱分析和初步鉴定。结论 建立了田和 Caski 细胞胞膜蛋白 2DE 图谱，并初步发现 LRC8D 、EDAR、MTXl 蛋白在宫颈癌前病变和宫颈癌细胞胞膜 中存在差异表达，为宫颈癌及癌前病变的诊断及癌变机制提供了帮助。 [关键词 1 日8 细胞 ;Caski 细胞;差异蛋白质纽 ;2-DE;质谱 【中图分类号] R 361 ? . 3 【文献标识码] A [文章编号] 1∞4-0501 (2011 )09-1336-03 Comparative Proteomiαanalysis of membranous proteios of immortalized cervical ceU and αrviω1 canαr cell. SONG Da-pi唔 I ，z.剧o YOI尊2. 1. The Third People￥ Hospital of M.ωnyang ， Mianyal略 ， Sichuan 621仪队2. C1wngqing Medicine U- nivers町 ， C1wngqing 4α刃而 ， China [ Abstract] 0均优tiVI刊.e To i尬den川t江均yd也ifferen臼e 眈阳t vical cωell ( H8) and cervical c 四ce町r cell (Caωski) associated w町it由h cωe凹町i臼1 c俑arcmog，阱:ene岱由s刽i总s b均Y t阳wo萨-dim阳e臼ns恼i沁onal ele倪ctrophoresis (2-DE) analysis. Meth创s The membranous proteins of H8 cell and Caski cell were separated by means of two-dimensional gel el优trophoresis ， then 由e selected differential protein spots were analyzed by matrix-assisted 1酣r de回rptionlionization time-of- flight mass spectrome町 (MAWI-TOF-MS) and SWISS-PROT database. Results 13 differentially expressed proteins spo恼 from these membranous proteins in H8 cells and Caski cells were 缸rther identified by MAWI-TOF-MS analysis and 3 of them were suc- C笛sfully identified and characterized. LRC8D( Leucine-rich repeat-containing protein 8D) 、EDAR( Tumor necrosis factor recepωr superfamily member EDAR precursor) 、Metaxin-l we陀 significanùy up-regulated in memhranous proteins in H8 cells. Condusion There differential expressions of memhranous proteins of may b