基因操作原理 基因操作原理-练习题.docxVIP

基因操作原理Genetic engineering has successfully transferred genes from eukaryotic cells into:Bacteria; B) plants; C) animals; D) all of aboveThe gene manipulation was born in:1970; B) 1972; C) 1973; D) 1976The first functional gene product from a chemically synthesized gene is:Growth honmore; B) somatostatin; C) insullin; D) interferonPhysical protection has four levels, the highest level is:P1; B) P2; C) P3; D) P4Which one of following are the basic type of phage λvectors?Insertional vectors; B) replacement vectors; C) both A and B; D) none of the aboveThe main advantage of cosmid over both plasmid and phage is:Cosmid can be used for cloning larger pieces of foreign DNA;Cosmid is dsDNA vector;Cosmid has cos site;Cosmid can be packed in vitroWhich of following characters is not the advantage of M13 series vector?It can be replicated via a double-stranded circular DNA (RF) intermediateBoth RF and single-stranded DNA will transfect competent E.coli cells to yield either plaques or infected colonies.There are no packaging constraints;All of aboveThe phasemids have been developed by containing:A part of the lacZ gene with pBR322A part of the lacZ gene withM13A part of the M13 genome with pUC8A part of the λ genome with pUC8Which of the following is not a method to obtain full-length cDNA clone?CAPture; B) oligo-capping; C) primer extension; D) 5’-RACECells transformed with pBR322 carring foreign fragment (no promoter activity) in the promoter region of TcR gene, its genotype is:ApRTcR; B) ApSTcR; C) ApRTcS; D) ApSTcSWestern-blot screening for a gene depends on :The clone gene expressed;The purified antibody binds only to the target protein;The target protein is DNA binding protein;Both A and B PCR screening belongs to:Sequence-dependent screeningImmunological screeningFunctional screening All of aboveRecombinational probe method for screening recombinant clones is:Based upon expressing vector;Based upon expressio