基于免标记电化学免疫传感的蔬菜中农药残留检测研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2014, Vol.30, No.10 基于免标记电化学免疫传感的蔬菜中 农药残留检测研究 韩恩，周立娜，闫景坤，蔡健荣，田苘榕，曾倪 （江苏大学食品与生物工程学院，江苏镇江 212013） 摘要：本文利用层层自组装技术构建了一种用于农药残留高灵敏检测的免标记电化学免疫传感器。该免疫传感器首先通过在天 然聚合物海藻酸钠修饰的玻碳电极上利用电化学方法原位聚合金纳米粒子，然后借助金纳米粒子与蛋白质抗体之间的较强吸附作用进 一步原位组装农药抗体，从而成功构建了免标记电化学免疫传感器。实验中以呋喃丹农药为模型，呋喃丹分子通过特异性的免疫反应 在抗体功能化电极表面生成免疫复合物，该复合物阻碍了电化学探针在电极表面的电子传递，从而减小了免疫传感器的电流响应，利 用电流响应的变化与农药分子浓度的关系可以实现呋喃丹农药的快速、高灵敏检测。在优化的实验条件下，该传感器对呋喃丹农药的 线性检测范围为 1~105 μg/L，检测限为 1 μg/L。同时，该免疫传感器还实现了多种蔬菜样品中呋喃丹农药残留的高灵敏检测。 关键词：免标记；电化学免疫传感；农药残留；呋喃丹 文章篇号：1673-9078(2014)10-268-273 DOI: 10.13982/j.mfst.1673-9078.2014.10.045 Label-free Electrochemical Immunosensor for Sensitive Detection of Pesticide Residue in Vegetables HAN En, ZHOU Li-na, YAN Jing-kun, CAI Jian-rong, TIAN Qing-rong, ZENG Ni (School of Food Biological Engineering, Jiangsu University, Zhenjiang 212013, China) Abstract: This study describes the application of a label-free electrochemical immunosensor that was designed for highly sensitive detection of pesticide residue using a layer-by-layer self-assembly technique. This electrochemical immunosensor was first constructed by placing self-assembling gold nanoparticles on the sodium alginate functionalized glassy carbon electrode (GCE) using in situ electrodeposition. Subsequently, further in situ assembly of the pesticide antibody was conducted by utilizing the strong adsorption between the gold nanoparticles and protein molecules in order to successfully construct the label-free electrochemical immunosensor. In the experiment, carbofuran was used as the pesticide model. An immune complex was produced by the specific immune