真核表达质粒pIRES-ST3GALⅠ的构建及其在大肠杆菌中的初步表达.pdfVIP

西南农重季簸 2015??28??5?? ?? S??mtllwe??Chi??J01Imal? ?鷏??? Sci?????? VoL28 ? 5 文章编号：??一??????—??—? DOI??10??16213??j??cnki??3cj????2015??05??083 ????????????pIRES??S??r3GALI?????? 及其在大肠杆菌中的初步表达 吴亚丽?保?熨?，武志丹??嗣蚊?，张剑??钚律鷋 ?．河南农业大学牧医工程学院。河南郑州???；??泄?抟揭┢芳嗖焖??本???? 摘要：构建?肴樘擒誵?，?煌僖核嶙R泼窱????基因的真核表达栽体????薌?，并诱导使其在大肠杆菌中高效表 ??????pGEM-??GAu????????????????PcR??????????????s????????????????????????????????????????????pIREs?? ?鐶?。经??⑺?┣泻筒獍都?ㄕ?泛螅?;?暄⊙粜灾刈榇螅?崾闳????Ｖ懿煌?ǘ鹊囊毂??鸫?肴樘擒?研?诱 导表达目的蛋白，并用?％??狿???ā＝峁?砻鳎?刈橹柿???—?畁??中的????蛄杏朐?Φ膕???氨基暖 序列同源性?．?ィ?胫淼耐?葱源???％，与人的同源性达?．?ァ?半乳糖苷??，?ね僖核嶙R泼竘在大肠杆菌中实现了 ??????????????????????????????????B????????a??2??3??????????????I(s13GALI)??????????????pII??S??ST3GALI?????????? 传代细胞建立重组传代细胞系奠定了基础。 关键词：?肴樘擒誨?。?煌僖核嶙R泼窱；基因克隆；原拨表达 ????????????s852??65+9??5????????????A Co??tmction? ????? ????? ?嬲?? pmES-Srl3 ?? and ??? PreUnliIIaI??E??ression?曰．?髓 ? ?出??，? Qi??1??WUZhi_d??1??PAN??．?㊣??琙?? ?蚰?琔 xin??8hen91?? ?．??? ? Hu8h??dry??ndVeteri??????He??A??cIllnIfmulli??ity??Hen??????sdmu450002??cIIi????2??clli??h??tIlte???蚽矗? D????Corltrol??BeUing???，???? ???????? ?? ??藁譭?∞?? ????．??? acid??ndha??(S13GALI)I??????karyo??????8i????0r??????? 1 ?鱞??? induced ??蔱 e??cient??re??ion?