油樟油副产物对细菌性皮肤致病菌的抑制作用.pdfVIP

第 32卷 第 1期 2014年 3月 四川农业大 学学报 Journal of Sichuan Agricultural University Vo1．32 No．1 M ar． 2014 doi：10．3969／j．issn．i000—2650．2014．01．009 油樟油副产物对细菌性皮肤致病菌的抑制作用 黄 彤 ，游 玲。，杜永华。，税之乎 ，邓 杰 ，魏 琴 (1．宜宾学院生命科学与食品工程学院／宜宾学院西南特色经济植物保护与利用重点实验室，四川 宜宾 644007； 2．发酵资源与应用四川省高校重点实验室，四川 宜宾 644007) 摘要 ：【目的】研究白樟油等几种油樟油副产物对 4种常见细菌性皮肤致病菌的抑制作用。【方法】采用滤纸片扩散 法和试管二倍稀释法测定 白樟油等几种油樟油副产物对 4种常见细菌性皮肤致病菌的最低抑菌浓度和最低杀菌浓 度 ，并通过平板菌落计数法绘制抑菌曲线。【结果】白樟油等几种油樟油副产物对大肠杆菌、变形杆菌、金黄色葡萄 球菌和痤疮杆菌有不同程度的抑制作用，属于浓度依赖型，其最低抑菌浓度(MIC)值在 3．13～12．5“L／mL，最低 杀菌浓度(MBC)值在 6．25～25 fL／mL。【结论1白樟油等几种油樟油副产物有较好的抑菌活性，具有治疗细菌性 皮肤病的潜力。 关键词：油樟油副产物；细菌性皮肤致病菌；抑制作用 中图分类号 ：$565．9；Q935 文献标 志码 ：A 文章编号 ：1000—2650(2014)O1—0053—06 Inhibiting Effects of By-products from Oil on Pathogenic Bacteria Cinnamomum iongepaniculaturn Causing Skin Infection HUANG Tong ，YOU Ling ，DU Yong—hua。，SHUI Zhi—hu ，DENG die ，WEI Qin (1．Life Science and Food Engineering Institute／Key Laboratory for Protection and Utilization of Characteristic Cash Plants in the Southwest China，Yibin University，Yibin 644007，Sichuan，China； 2．Key Lab of Fermentation Re— source and Application of Institutes of Higher Leaming in Sichuan，Yibin 644007，Sichuan，China) Abstract：[Objective]The aim of the study was to investigate the inhibiting effects of several by— products extracted from C．1ongepaniculatum oil on four pathogenic bacteria causing skin infec— tion．【Method]The minimum inhibitory concentration (MIC)and bactericidal concentration (MBC)of the by-products to pathogenic bacteria were detected with filter paper diffusion and tube dilution methods and the time—kill curve was drawn using flat colony counting method．[Results] The by—products extracted from C．Z0 g 户口 f Z口 oil including white camphor oil had different inhibiting effects against Escherichia coli，Proteus species，S￡口 Z0c0cc s aureus and Propi— onibacteriurn acnes and inhibiting effect was concentration—dependent． The MICs ranged from 3．13~L／mL to 12．5 L／mL and MBCs ranged from 6．25／,L／mL to 25 L／mL．[Conclusion] These by～product