细胞膜质子泵基因超表达促进水稻种子萌发.pdfVIP

中国水稻科学 (ChinJRiceSci)，2012，26(6)：651—655 http {{w ．ricesci．en DOI：10．3969／j．issn．1001—7216．2012．06．003 651 细胞膜质子泵基因超表达促进水稻种子萌发 刘赣 张明超 曾后清 ， 朱毅勇 ， 孙淑斌 徐国华 (1南京农业大学 资源与环境科学学院农业部长江中下游植物营养与肥料重点实验室，江苏 南京210095；2杭州师范大学生命与环境 科学学院，浙江 杭州 310036； 通讯联系人，E-mail：yiyong1973@njau．edu．cn) OverexpressionofaPlasmaM embraneH十一ATPaseGenePromotesRiceSeed Germ ination LIU Gan1，ZHANGM ing—chao ，ZENGHou—qing1·2，ZHU Yi—yong1， ，SUN Shu—bin1，XU Guo—hua1 (1KeyLaboratoryofPlantNutritionandFertilization inLow-MiddleReachesoftheYangtzeRiver，MinistryofAgriculture， CollegeofResourcesandEnvironmentalSciences，NanjingAgriculturalUniversity，Nanjing210095，China；2CollegeofLifeand EnvironmentalSciences，HangzhouNormalUniversity，Hangzhou310036； Correspondingauthor，E-mail：yiyon 973@njau． edu．cn) IIU Gan，ZHANG M ingchao，ZENG Houqing，eta1． ()verexpression ofaplasma membrane H 一ATPasegene promotesriceseedgermination．ChinJRiceSci，2012，26(6)：651—655． Abstract：ToverifythedirecteffectofH 一ATPaseontherootcellelongation，theT2generationseedsoftherice plasmamembraneH 一ATPasegeneOsA8 (AJ440219) overexpressed transgenicmaterials (0E1)wereused as samples．Firstly， the differences in seed germination rate and the growth rate of primary roots between the overexpressedline and the wild—type weredeterm ined． Secondly， the activitiesofH 一ATPase in the root cel1 membranesisolated by two-phaseseparation method were compared and then the OsA8 gene expression atthe transcriptional1evelwasdetermined．TheproteinconcentrationofH 一ATPaseofthesetwomaterialswasanalyzedby W esternblot．TheresultsshowedthatnotonlythegerminationratebutalsothegrowthrateofprimaryrootsofOE1 werehigherthanthatofwild—type．Inaddition，()E1hasmuch higheractivityoftherootplasmamembraneH 一 ATPasethanwild—type．TheexpressionofOsA8 geneandproteinconcentrationof()E1wasalsohigherthanthatof wild—type．Theresultsindicatedthattheovere