内源性TGFβ1和TL1A对高糖所致血管内皮细胞凋亡的影响.pdfVIP

The effects of endogenous TGFβ1 and TL1A on high glucose-induced apoptosis in endothelial cell [ABSTRACT] Background Excessive(Accelerated) endothelial cell apoptosis is a critical event in the process of diabetes-associated vascular disease. It’s mechanism has not yet been clarified. The changes of cytokines synthesis and biological activity have been shown to play a potentially important role in high glucose-induced cell apoptosis. Both TGFβ1 and TL1A were shown to mediate apoptosis. TGFβ1 is considered a key modulator of endothelial cell apoptosis, there is, however, little evidence establishing a direct link between the effects of high glucose on apoptosis and autocrine action action of TGFβ1 in endothelial cells. It remains contentious whether exposure to high glucose does induce TGFβ1 production by endothelial cells. TL1A, as a recently newly discovered membre of tumaor necrosis factor (TNF)cytokine family, has been repored to play an important role in endothelial cell apoptosis. However, no study explore wether TL1A involves in the process of diabetes-associated vascular disease. Objective To observe the TGFβ1 and TL1A expression in human umbilical vein endothelial cell (HUVEC) under the high glucose(HG) condition, and to investigate the effects of endogenous TGFβ1 and TL1A on high glucose- induced apoptosis. Methods 1. HUVEC were exposed to different concentrations of glucose (5.6, 11.2, 22.4, 33.6mM)for 24 hours or to 22.4 mM glucose for different time (0h, 6h, 12h, 24h, 48h). 5.6 mM glucose plus 16.8mM mannitol as an osmotic control. TGFβ1 and TL1A expression was evaluated by RT-PCR and Western Blotting. 2. Cultured HUVECs were divided six groups:①5.6mM glucose(NG) group ②NG and TGFβ1 group ③NG and TL1A group ④22.4mM glucose (HG) control group ⑤HG and TG