-Activated protein -1 in the diet of retinal light damage in the protective effect of taurine expression of.docVIP

 PAGE \* MERGEFORMAT 15 -Activated protein -1 in the diet of retinal light damage in the protective effect of taurine expression of [Abstract] To investigate the protective effect of taurine diet molecular mechanism of retinal photochemical damage. Methods 30 Sprague-Dawley rats were fed with AIN-93 standard feed or add 4% taurine dietary intervention in 15 d, giving (3 000 ± 200) lux continuous light 24 h, morphology was observed by measuring the thickness of the retina outer nuclear layer , electrophysiological retinal function were detected, Western blot or immunohistochemistry to detect activated protein -1 (actor protein-1, AP-1) subunit c-fos/c-jun protein expression, fluorescence quantitative PCR to detect expression of its mRNA . The results of light can reduce the retinal outer nuclear layer thickness, reduced ERG a-wave and b-wave amplitude, increased AP-1 protein and mRNA expression of 4% dietary taurine reduced the level of transcription and translation of the expression of AP-1 , effective protection of retinal photochemical damage. Conclusion transcription factor AP-1 in retinal light damage protective dietary taurine play an important role. [Keywords:]-activated protein -1 taurine retinal light damage Abstract: Objective To investigate the underlying molecular mechanism involving in dietary taurine protecting retina from photochemical damage.Methods Thirty Sprague-Dawley rats fed with or without 4% taurine under AIN-93 diet for 15 d were persistently exposed to fluorescent light (3000 ± 200 lux) for 0-24h.The thickness of outer nuclear layer (ONL) of retinas was measured with morphometry.The retinal function was detected with electrophysiological technique.The expression of actor protein-1 (AP-1) subunits c-fos / c -jun protein was analyzed with Western blot or immunohistochemical method, and their mRNA expression determined with quantitative real-time PCR (qRT-PCR). Results There was a decrease in thickness of ONL, and in amplitude of a-a