-Mediated CTLA4Ig and Iκ Bα adenovirus vector of the burn grafting of creating skinned.docVIP

 PAGE \* MERGEFORMAT 6 -Mediated CTLA4Ig and Iκ Bα adenovirus vector of the burn grafting of creating skinned [Abstract] Objective To study the reorganization of CTLA4Ig and Iκ Bα adenovirus vector to extend the burn record skinned graft survival. Methods Wistar rats were 36, were randomly divided into three groups, Group 1: normal control group, Group 2: liquid at room temperature with AdCTLA4Ig soak transfection 1h, 3 group: Adv CD40LIg-IRES2-CTLA4Ig transfection soaking liquid at room temperature 1h. Conventional back skin preparation after anesthesia, in an area of 4cm * 4cm Tu 3% napalm 2ml, burning 20s, back skin of rats Ⅲ degree burns, after three days at the burn will be prepared by 1.0cm * 2.0cm size of the rectangular skin flap in the wound, pressure bandage. Observation of recombinant adenovirus vector transfected grafts after transplantation in rat burn wound back the survival of human skin fault situation. Results adenovirus vector could be extended burn wounds in rats transplanted human skin, the survival time of fault. Conclusion recombinant adenovirus can be used to extend the burn wound allogeneic skin graft survival. [Keywords:] CTLA4Ig; Iκ Bα ; adenovirus, recombinant; allograft Prolonging the survival of transplanted xenoskin by transfected the CTLA4Ig and Iκ Bα locally with recombinant adenovirus vector in rats Abstract: Objective To observe the effect of recombinant expression vector of CTLA4Ig and Iκ Bα on the survival time of xenoskin graft on burn wound.Methods A total of36Wistar rats established as Ⅲ degree burn on the back were randomly divided into3groups (n = 12in each group): normal control (the xenoskin without any special treatment), AdCTLA4Ig group (the xenoskin was transfected with AdCTLA4Ig), and Adv CD40LIg-IRES2-CTLA4Ig group [the xenoskin was transfected with the recombinant adenovirus expression vector of CTLA4Ig and Iκ Bα (Adv CD40LIg-IRES2-CTLA4Ig ) that was constructed by means of homorecombination]. After