1 HIF senile dementia model rats in the hippocampus of.docVIP

 PAGE \* MERGEFORMAT 11 1 HIF senile dementia model rats in the hippocampus of Of: Liu Lingyun Ma Yuanyuan Li Xin Chai Xiqing [Abstract] Objective To observe the senile dementia (AD) rat behavior, neuronal apoptosis and hypoxia inducible factor (HIF) 1 @ expression level. Methods Construction of intracerebroventricular injection of A25 ~ 35 AD rat model Morris water maze test spatial learning and memory in rats by flow cytometry rate of neuronal apoptosis, Western blot detection of hippocampal protein expression of HIF 1 @. Results Morris water maze test showed that the average AD group escape latency was significantly longer (P lt;0.05), flow cytometry analysis showed, AD group the percentage of neuronal apoptosis compared with normal control group was significantly higher (P lt;0.05), Western blot analysis showed, AD hippocampal neurons HIF @ 1 protein levels compared with normal control group was significantly higher (P lt;0.05). Conclusions ICV injection of A25 ~ 35 AD rat model was successfully constructed and hippocampal neurons increased the level of expression of HIF 1 @. [Keywords:] Alzheimer’s disease; hypoxia-inducible factor 1 @; amyloid protein; hippocampus Also known as senile dementia Alzheimer’s disease (AD), the central nervous system is a chronic, progressive degenerative disease, cognitive dysfunction, senile plaques, neurofibrillary tangles, is characterized by granular degeneration (1) . AD pathogenesis is not fully understood, amyloid (A) theory that A is the initiating factor in AD pathogenesis, A deposition led to a large number of abnormal nerve cell death, loss and other symptoms appear intelligent. but abnormal deposition of A is still a small area nerve cell survival, some scholars have also successfully screened from A toxicity that can withstand PC12 cell line (2,3), Semenza et al (4) that the mechanism of survival of these cells may be related to glucose metabolism and hypoxia inducible factor (HIF ) For, worthy of f