32 cases of children in clinical research unrelated umbilical cord blood transplantation.docVIP

 PAGE \* MERGEFORMAT 19 32 cases of children in clinical research unrelated umbilical cord blood transplantation Abstract In this study, Shandong Province, cord blood hematopoietic stem cell bank from August 2001 to June 2004 provided 32 cases of children in unrelated umbilical cord blood transplantation (URCBT) were retrospectively analyzed to observe unrelated umbilical cord blood hematopoietic stem cell transplantation (UCBT ) the efficacy and complications. Routine for cord blood collection, separation, storage, detection, freezing and thawing, and infusion. Using PCR-SSO, PCR-SSP, SBT detection of HLA-A, B, DR. The results showed that: 32 cases of children suffering from blood diseases, with a median age of 8 (2-15) years of age; median weight of 31.5 (14-55) kg, including acute lymphoblastic leukemia (ALL) 13 cases were high-risk type; acute myeloid leukemia (AML) 9 cases, including M1 1 Li, M2 4 Li, M4 3 Li, M5 1 cases; chronic myeloid leukemia (CML) 2 cases; severe aplastic anemia (SAA) 3 cases; acute mixed leukemia (HAL) 3 cases; non-Hodgkin’s lymphoma in 2 cases. Recipient for HLA identical sibling is 10 cases, a locus-mismatched in 16 cases, two-loci-mismatched for the 6 cases. Coincide for the recipient ABO blood type 12 cases, 20 cases do not coincide. Enter nucleated cells (TNC) 5.57 (2.16-12.3) × 107/kg, CD34 cells, 1.78 (0.85-5.92) × 105/kg. To adopt a cyclophosphamide (Cy) and total body irradiation (TBI)-based Cy / TBI conditioning regimen in 4 cases, in order to busulfan (Bu) and cyclophosphamide (Cy)-based Bu / Cy pretreatment program of 21 cases, seven cases of pre-processing in other programs. GVHD prevention with cyclosporin A use-based programs. The results showed that: 32 patients, 20 patients (62.5%), white blood cells to implant standards, ANC ≥ 0.5 × 109 / L, the median time of 17 (9-38) days; Plt ≥ 20 × 109 / L a median time of 42 (18 -102) days. Ⅱ - Ⅳ degree and grade Ⅲ - Ⅳ cumulative incidence of acute GVHD were 35% and 1