370 children in the laboratory detection of Mycoplasma pneumoniae.docVIP

 PAGE \* MERGEFORMAT 3 370 children in the laboratory detection of Mycoplasma pneumoniae Author: Chen Jianwei Chen Weiyu HU Xiu-Hua Chen Ying Xia Jin-Wei Tu Xiuju [Keywords:] Children Mycoplasma Pneumonia laboratory testing Mycoplasma pneumoniae (MP) is a class of a lack of cell walls, showed a high degree of morphological diversity, through sterilization filter, in the absence of life, growth and reproduction of the medium can be the smallest prokaryotic cell type bacteria, a pathogen causing respiratory tract infection. Mycoplasma pneumonia in children in hospital laboratory research work began in 2005, after two years of research and work, the research group has accumulated a large number of experimental data and analysis of information, these are as follows. One specimen and equipment 1.1 The specimen sources from October 2006 to October 2008, hospital outpatient and hospitalization of 0.5 ~ 13 years old children with 387 cases, the main symptoms are coughing. Wipe with sterile cotton swab directly to the posterior wall of the pharynx, tonsil, pseudomembrane at the edge or mouth ulcers for culture and polymerase chain reaction (PCR) detection; respiratory tract infections for more than 4d cases of venous blood for serological tests. 1.2 Reagent and instrument applications Roche (Lightcycle) real-time fluorescent PCR to measure the MP-DNA, MP-PCR kits for the Zhongshan daan company’s products; Application Finland Thermo Labsystems ELISA plate washer determination of MP-IgM, reagents for Shanghai China and Thailand biological Engineering Company; gelatin particle agglutination reagent for the Japanese rich Guthrie will Europe Ltd. production; medium departments self-made; gene sequencing Dean commission medical examination center in Hangzhou. Two detection methods 2.1 Real-time fluorescence PCR method is strictly enforced by normative standards, to F: 5’CCAACCAAACAACAACGTTCA-3 ‘, R: 5’ACCTTGACTGGAGGCCGTTA-3’ as primer, primer P1 gene region is located