5 anthraquinone derivatives and application of simultaneous determination of.docVIP

 PAGE \* MERGEFORMAT 12 5 anthraquinone derivatives and application of simultaneous determination of Of: Lvhui Ying, Zhao Chenxi, LIANG Yi-Zeng, Wu Hai [Abstract] Objective To establish the simultaneous determination of 5 medicinal rhubarb anthraquinone derivatives (aloe-emodin, rhein, emodin, rhein and emodin content of phenol method, optimization of ultrasonic extraction of anthraquinone process. Methods RP-HPLC, column: Waters C18 column (250 mm 4.6 mm, 5 m, mobile phase: methanol - acetonitrile -0.01% phosphoric acid (3:5:2), column temperature: 25 , flow: 1.0 ml / min, detection wavelength: 225 nm. The results were 5 anthraquinones has a wide linear range and showed a good linear relationship (R2 = 0.998 7 ~ 0.999 7, the average recovery was 92.79% ~ 99.28% day precision was 0.65% ~ 1.71%, inter-day precision was 2.52% ~ 3.46%. Conclusion The method was successfully applied to rhubarb Optimization of extraction of active substances, according to the established method measured content of anthraquinone 5 determine the best rhubarb anthraquinone ultrasonic extraction conditions were: 70% ethanol, solid to liquid ratio of 1:30 (g: ml), ultrasonic extraction 2 times extraction 20 min. [Keywords:] extraction, rhubarb, RP-HPLC, anthraquinone Abstract: ObjectiveTo develop a method for the simultaneous determination of five anthraquinone derivatives (aloe-emodin, rhein, emodin, chrysophanol and physcion) in rhubarb, and to optimize the ultrasonic extraction conditions.MethodsRP-HPLC on a Waters C18 column (250 mm 4.6 mm, 5 m) was used. The mobile phase was methanol-acetonitrile-0.01% phosphoric acid solution (3:5:2), at a flow rate of 1.0 ml / min, and the detection wavelength was 225 nm. ResultsThe linear ranges of the five free anthraquinones were wide, showing good linear correlations with correlation coefficients (R2) of 0.9987 to 0.9997. The average recoveries were from 92.79% to 99.28%. While the intra-day and inter-day precision were 0.65% ~ 1