A copy of Chinese medicine snake venom production of DNA molecular identification of.docVIP

 PAGE \* MERGEFORMAT 16 A copy of Chinese medicine snake venom production of DNA molecular identification of [Abstract] Objective-PCR identification of the source of dried snake venom species, and genomic DNA-extraction and extraction process in a number of issues need to pay attention to analysis and discussion, but also a number of different lengths for the purpose of gene amplification primers validate the use of effects. Method from a copy has been saved seven years of production dried snake venom extract total DNA, and to it as a template for PCR amplification, sequencing and sequence analysis. The results using this method can be extracted from snake venom to be trace amounts of total DNA, and successfully amplified by 0.45 ~ 1.18 kb fragment range of different lengths; sequencing results to the NCBI, the homology with the GenBank sequence BLAST alignment . Conclusion The samples from cobra snake venom, and most likely by a number of different sub-species or haplotypes formed. The technology may be used to promote the source of animal venom accurate and direct identification of. [Keywords:] snake venom; molecular identification; mitochondrial DNA; DNA sequencing Abstract: ObjectiveTo identify dried snake venom by PCR. MethodsTo extract DNA and subsequent sequencing of the mitochondrial genes from a snake venom that had been preserved for nearly 7 years.ResultsMinimal genomic DNA could be extracted from the crude venom by this method, and from which different length of target regions that ranged within 0.45 ~ 1.18 kb were amplified successfully. All the sequences were submitted to NCBI and compared against their homologous sequences in the GenBank database. ConclusionSequence alignment analyses approved that Naja naja was its most probable original specie, and this sample could be consisted by some different subspecies or haplotypes. This approach offers a straight forward method for direct and accurate identification of animal crude venoms. Keywords::