A labeling efficiency for the bio-chip research methods.docVIP

 PAGE \* MERGEFORMAT 12 A labeling efficiency for the bio-chip research methods Of: Wang Zhiwei, Ma Wenli, Xia Wei, Qiong Yang, Zheng Wenling [Abstract] Objective self-hybridization of the microarray samples and laboratory markers to detect the efficiency of quality control. Methods for Extracting RNA human leukemia K562 cells, will be divided into two equal parts such as nucleic acid samples, application of universal primers were labeled Cy3 and Cy5 fluorescent material, and preparation of the K562 and chip hybridization, after hybridization probe by comparing Cy3 and Cy5 fluorescent signal intensity of labeling efficiency and hybridization of samples for experimental analysis and quality control processes. Results chip probe showed hybridization Cy3 and Cy5 for the same amount of yellow fluorescence after overlap, because the same sample is the same amount of nucleic acid, thus proving the efficiency of the samples are in good agreement marker. Conclusion way through its own hybrid the same samples to compare the two fluorescent markers signal strength, the quality of the chip itself to detect, analyze the efficiency of marker tags can be used in quality control of microarray experiments. [Keywords:] bio-chip; marker; hybridization Abstract: Objective To investigate the effect of self hybridization method of analyzing samples labeling for microarray experiment. Methods The total RNAs were extracted and purified from K562 cells to synthesize double stranded cDNAs by reverse transcription. Then two homologous samples were labeled with two different fluorescent dyes Cy3 and Cy5. And the labeled samples were examined with the printed microarray. Results With the same quantity of fluorescence Cy3 and Cy5 overlap, the microarray image showed almost yellow color. And the self hybridization method could be used to control the fluorescence quality. Conclusion The method that used self hybridization method to obtain quality data set from a microarray experimen