A novel about climacteric syndrome and analysis of cell model.docVIP

 PAGE \* MERGEFORMAT 11 A novel about climacteric syndrome and analysis of cell model Of: Lu Jinfu Hong Min Quan Du Ping Zhu [Abstract] Objective To establish the climacteric syndrome cell model. Methods in the adult rat granulosa cells added to a final concentration of 30%, 20%, 10% inactivated and non-inactivated perimenopause, adulthood, childhood of serum, granulosa cells determined by MTT growth. results in non-inactivated serum, the final concentration of perimenopause on the granulosa cells in serum showed a significant catalytic role, and adulthood, infancy large rat serum, only 10% of the group on the granular cells promoted significantly. All inactivated mouse serum on the growth of granulosa cells have good support for the role, including a final concentration of 10% rat serum perimenopause granulosa cells support the growth of the role of the minimum. Conclusion 10% of perimenopausal inactivated serum from adult rat granulosa cells co-culture system can be used for perimenopausal syndrome drug high-throughput screening platform. [Keywords:] climacteric syndrome, cell model, granule cells Perimenopausal women, is the decline of ovarian function to the complete disappearance of a transition period, the long-term estrogen levels in the lower state, giving rise to a variety of clinical symptoms. Perimenopausal ovarian syndrome is a pathological secretion of real recession Therefore, the current copy of the pathology model is mainly by surgery, drugs and other means to cause the attenuation or loss of ovarian function, such as the type of castration, castration plus traditional Chinese medicine-based, X ray damage type, alcohol type, etc. (1 ~ 6). These models side reactions in different clinical features of menopause some, but not yet perimenopausal syndrome cell model reported. In this paper, different concentration of serum growth period, and adult co-cultured rat granulosa cells was observed serum levels of different growth in adult rat g