A rabbit cyclosporine intraocular sustained-release microspheres release study.docVIP

 PAGE \* MERGEFORMAT 16 A rabbit cyclosporine intraocular sustained-release microspheres release study Abstract] Objective To establish a rabbit aqueous humor of cyclosporin A by high performance liquid determination of sustained release microspheres, this study investigated different dose in the aqueous humor of rabbit pharmacokinetic properties, in order to prevent after-cataract drug to provide the theoretical basis. Methods lens capsule of rabbit eyes with extracapsular cataract extraction, injection of the left eye anterior chamber and capsular bag cyclosporine microspheres, blank microspheres given the right eye as control, monitoring by high performance liquid chromatography of rabbit eyes 4 weeks after drug administration room A water concentration of cyclosporine. column RP C18 silica gel column as the column temperature is 60 , mobile phase of acetonitrile water (67:33), detection wavelength 210nm. The results of rabbit aqueous humor of cyclosporin A, concentration of 0.13-1.25mg / L was linear within the range (r = 0.9951), the minimum detectable concentration of 0.13mg / L, recovery of 95.91%, day and day precision were less than 5.0%. Microspheres The drug release rate is moderate, rabbit anterior chamber in 2 weeks after administration to maintain a high concentration of high and low dose, the absorption of cyclosporine A half-life (t1/2Ka) and plasma clearance rate (CL) Upon examination no significant difference between the area under the curve (AUC) and peak concentration (Cmax) and the linear correlation between dose. Conclusion cyclosporine A sustained release microspheres in the rabbit eye has a higher bioavailability, After the experimental period to suppress the effective concentration of cataract. [Keywords:] cyclosporin A, sustained release microspheres, aqueous, pharmacokinetics ABSTRACT: Objective To study the pharmacokinetic characters of cyclosporin A (CsA) in aqueous humor in rabbit after implanting different dosages of CsA in