A swelling on the HPLC determination of serum levels of consumption and a glycoside content of Fritillaria Turkey.docVIP

 PAGE \* MERGEFORMAT 9 A swelling on the HPLC determination of serum levels of consumption and a glycoside content of Fritillaria Turkey OF: John Cui Ming, Cheng Huiping, Liu Ximing, Zhu Xiaoyun [Abstract] Objective To establish a BZX preparations and oral administration in rats after elimination of serum goiter Turkey Fritillaria A method for the determination glycosides. Methods RP-HPLC method, a methanol extraction BZX preparation, serum samples were C18SPE solid phase extraction column (3 mL, 200 mg, 60 μm) treatment, methanol elution, column: Diamonsil C18 (250 mm × 4.6 mm, 5 μm) and Easyguard guard column, mobile phase: methanol - water (65:35 ), detection wavelength: 214 nm, flow rate: 1 mL / min. Results A BZX Fritillaria preparations and serum glycoside A Turkey’s recovery were (103.49 + -3.49)%, (104.70 + -4.69)% , Tubeimoside at 1.18 ~ 88.5 μg / mL concentration range of good linearity. each containing 1 g preparation Tubeimoside 995.3 μg, given orally after a BZX a serum concentration of glycosides Fritillaria Turkey 3.19 μg / mL. Conclusion The method is fast, simple and accurate preparation and serum for the determination of a glycoside content of Fritillaria Turkey. [Keywords:] A BZX, Tubeimoside, solid phase extraction, HPLC, containing serum, determination Keywords:: Jiazhongxiao, Tubeimoside Ⅰ, SPE, RP-HPLC, drug containing serum, determination A BZX prescription by the Prunella, Fritillaria and other components of soil, the concentration of an appropriate process into 3 g / mL of the original ingredients of the extract agents. Animal and clinical trials proved that the agent has good phlegm Sanjie, treatment, thyroid function The role of hyper goiter. But its effect and the mechanism is not clear, so we used the method of serum pharmacology, Rats were observed after administration of the formulations containing serum on the proliferation of thyroid cells to clarify Treatment of hyperthyroidism in this mechanism. soil Fritillari