A first derivative spectrometry phenol content of conductive gel.docVIP

 PAGE \* MERGEFORMAT 5 A first derivative spectrometry phenol content of conductive gel Author: Gu Wei Ying Zhang Heping Cao Qinghua [Abstract] Objective: To establish a conductive gel method for the determination of phenol. Methods: The first order derivative spectroscopy in the (279 ± 1) nm wavelength of the direct determination of phenol content. Results: The phenol 25.47 ~ 45.85μ g * ml-1 concentration range, a good linear relationship, the correlation coefficient r = 0.9999, average recovery was 99.43%, RSD 0.36%. [Keywords:] a first-order derivative spectrophotometry determination of conductive gel phenol Conductive gel containing phenol, glycerine, sodium chloride and sodium carboxymethyl cellulose, etc., for heart, EEG, and electric shock treatment described conductive contacts, to close contained in the ‘Shanghai hospital preparation manual’ [1] Article 3 version, but only identification of its quality control, rather than make a determination. Related to derivative spectrophotometry in pharmaceutical analysis has long been reported [2], this study used a first derivative spectrometry phenol content, method is simple and accurate. An instrument and reagent Shimadzu 2450 UV spectrophotometer; phenol (Xiamen Dongfeng Pharmaceutical Co., Ltd., batch number 0507011); sodium chloride and glycerol, in line with the provisions of the Chinese Pharmacopoeia; conductive gel (hospital self-control, batch number 051123,060112,060320 ); sodium carboxymethyl cellulose as a chemically pure. 2 Methods and Results 2.1 Absorption spectra of drawing Fine said the phenol 0.9959g, allows the dissolved water, and quantitatively diluted to a concentration of 29.8770μ g.ml-1 solution. The other other ingredients prepared according to the proportion of prescriptions in aqueous solution, water as a blank, at 200 ~ 400nm wavelength range of the above-mentioned four kinds of solutions for scanning, respectively, shown in Figure 1. And the mapping of a first-order der