Abies shikimic acid in the extraction and determination of a preliminary study.docVIP

 PAGE \* MERGEFORMAT 12 Abies shikimic acid in the extraction and determination of a preliminary study Study: Forest in, Ma Lian move, Liu, Zhuoyu Juan, Zhang Guiping, ginger exhibition, Guo Hu, Yuan with [Abstract] Objective To establish Abies in the shikimic acid extraction process, and establish the determination method. Methods water extraction - alcohol precipitation method to extract shikimic acid, shikimic acid by HPLC method for the determination of the content. The results of shikimic acid in the 5 300 g mL-1 range, quality, concentration and peak area showed a good linear relationship (r = 0.999 3), the average recovery was 94.5%, RSD = 2.5% (n = 5). shikimic acid yield was 0.51 % (puritygt; = 96%). Conclusion The extraction process is simple; determination method is simple, accurate, reproducible and can be used for Abies in the determination of shikimic acid. [Keywords:] Abies; shikimic acid; extraction technology content; (Shool of Chinese Indigenous Medicine, Chongqing Medical University, Chongqing 400016, China) Abstract: Objective To establish methods for the determination and extraction of shikimic acid from Abies fargesii Franch.Methods Shikimic acid was extracted with H2O, and HPLC was employed to determine shikimic acid.Results The linearity of shikimic acid was good in the range of 5-300 g mL-1 (r = 0.999 3), and the average recovery was 94.5% (RSD = 2.5%, n = 5). The yield of shikimic acid was 0.51% (puritygt; = 96%). Conclusion The extraction technology is simple and convenient.This method is convenient, rapid and accurate, and can be used for the determination of shikimic acid in Abies fargesii Franch. Keywords:: Abies fargesii Franch; shikimic acid; extraction process; determination Now mainly comes from shikimic acid from star anise magnolia (Fructus anisi stellati), the anti-bird flu and a flu drug oseltamivir phosphate (Oseltamivir Phosphate) is an important precursor [1], so of shikimic acid has an important new