Abnormal Savda maturity of total flavonoids and total phenolic agents Determination.docVIP

 PAGE \* MERGEFORMAT 18 Abnormal Savda maturity of total flavonoids and total phenolic agents Determination Author: Murat Kezza clothing Buick Abudureyimu jade southern Jiangsu Kazakhstan Murat Gupur [Abstract] Objective To detect the abnormal Savda mature agent of total flavonoids and total phenolic content. Methods rutin as reference substance, using NaNO2-Al (NO3) 3-NaOH colorimetric determination of total flavonoids; to gallic acid as reference substance, using Folin-Ciocalteu colorimetric determination of total phenol content. Results of Rutin concentration of 0.011 ~ 0.130 mg / ml range a good linear relationship, the lowest detection limit was 0.06 μ g / ml, the lowest limit of quantification of 0.18 μ g / ml. The method recovery was 95.1% ~ 97.5%, precision 1.34%, water extract and ethanol extract of total flavonoids contents were 16.2 mg / g and 19.0 mg / g. Gallic acid concentration of 1.1 ~ 7.7 μ g / ml range a good linear relationship, the minimum detection limit of 0.01μ g/ml, the lowest limit of quantification of 0.03 μ g / ml. The method recovery was 99.5% ~ 104.8%, RSD 3.39%, water extract and ethanol extract of total phenol content were 38.28 mg / g and 21.54 mg / g. Conclusion The two methods sensitivity, accuracy, strong, high precision, good repeatability, respectively, can be used as agents of Abnormal Savda maturity of total flavonoids and total phenolic contents were determined. [Keywords:] Abnormal Savda mature agent phenol content determination of total flavonoids Abnormal Savda mature agents are commonly used Uighur medicine compound preparation, from Cordia Cordia dichotoma Forst., Jujube Ziziphus jujuba Mill., Licorice Glycyrrhiza uralensis Fisch., Bugloss Anchusa italica Retz., Fennel Foeniculum vulgare Mill., Euphorbia humifusa Euphorbia humifusa Willd. and so on medicinal composition for the treatment by the Abnormal Savda caused by cancer, diabetes and cardiovascular disease. Modern research has confirmed that abnormal Savda