Acellular nerve modified chemical preparation methods of experimental study.docVIP

 PAGE \* MERGEFORMAT 10 Acellular nerve modified chemical preparation methods of experimental study Author: Wang champion, Sun Ming Xue, Lu Shi-bi *, Xu Wenjing, Zhao Bin, Peng Jiang, ZHANG Li, HUANG Jing-xiang 【Abstract】 Objective] To improve the chemical extraction of acellular allogenic nerve preparation, prepared by complete removal of cells and myelin sheath, nerve bodies well-preserved acellular nerve grafts. [Methods] SD rats were 14, take bilateral sciatic nerve (total 28), divided into 3 groups for chemical extraction to the cell treatment: Sondell method group (10), modified method group (10) and the control group (8 ). Sondell method group used for extraction agent for the Triton X-100 and sodium deoxycholate; modified method group used for extraction agent for the Triton X-200, SB-10 and SB-16; the control group did not carry out chemical treatment. HE staining after treatment nerve lines, fast blue staining, immunohistochemical staining basement membrane elements and environmental scanning electron microscope, and from cell to the extent of grading and structural integrity of the myelin sheath stain three comprehensive evaluation. [Results] The physical properties of acellular allogenic nerve, the improved preparation of the acellular nerve toughness, elasticity slightly better than Sondell France; HE staining showed that two kinds of methods to cell effects are good, but the modified method of neural structures destroy small; fast blue staining, immunohistochemical staining basement membrane elements, environmental scanning electron microscope results show that the improved method in the removal of the myelin sheath, basement membrane elements for the preservation and the preservation of neural structures are superior to Sondell France ; integrated quality rating results show that the two kinds of methods similar to cellular effect of the (P1gt; 0.05), myelin removal and preservation of neural structures is better than Sondell Act Im