Activin A focal cerebral ischemia and its receptor ActRA Expression and significance of.docVIP

 PAGE \* MERGEFORMAT 8 Activin A focal cerebral ischemia and its receptor ActRA Expression and significance of Of: Mang Jing He Jinting Zong Guo Hongliang Shaoyan Kun Xu Zhongxin Xu Guihua tree 1 [Abstract] Objective To investigate cerebral ischemia activin A (Act A) and its receptor A (ActR ) and significance of the expression changes. Methods intraluminal middle cerebral artery occlusion (MCAO) model , by light microscopy, immunohistochemistry to detect rat infarct volume after focal cerebral ischemia, pathological changes, immunohistochemical staining Act A, ActR A immunoreactive expression. The results have a very low level of normal tissue Act A, ActR A expression, immunohistochemistry showed that 3 h after ischemia in the surrounding area Act A, ActR A number of positive cells was significantly higher than sham operation group (P lt;0.05), and with the extension of ischemic time was gradually increased and peaked at 24 h. Conclusion focal cerebral ischemia can induce the endogenous expression of Act A neurons, and may signal through specific transmembrane receptor ActR A target-derived role to play. [Keywords:] Focal cerebral ischemia; activin A; activated receptor [Abstract] Objective To investigate expression of Activin A and ActR A in rat after focal cerebral ischemia.Methods Focal cerebral ischemia was induced by permanent middle cerebral artery occlusion (MCAO) in the rat, injury and infarction volume in brain tissue were observed by HE stain , Activin A and ActR A protein expression was measured by immunohistochemistry.Results Activin A and ActR A protein expressed at a very low level in sham operation group, a strong up regulation in cortical areas next to the infarct was detected 3 h after MCAO (P lt;0.05 ). Expression of Activin A and ActR A increased in response to cerebral ischaemia and reached acme 24 h after MCAO.Conclusions The expression of Activin A and ActR A is gradually increased after focal cerebral ischemia, whic