Acupuncture on Spleen Zusanli TGF-β in asthmatic rats and the concentration of GM-CSF.docVIP

 PAGE \* MERGEFORMAT 16 Acupuncture on Spleen Zusanli TGF-β in asthmatic rats and the concentration of GM-CSF Of: Wu Zhaoli, Liuzi Li, ZHANG Qing-rong [Abstract] Objective To investigate the deficiency in asthmatic rat transforming growth factor β (TGF-β), granulocyte - macrophage colony-stimulating factor (GM-CSF) concentration and the regulation of acupuncture effect of the full three years. Methods TCM Spleen Animal models and animal models of asthma Western compound modeling methods, certificate combined rat model of spleen deficiency asthma, take the full three years of acupuncture method for treatment. by double antibody sandwich enzyme-linked immunosorbent assay (ELISA) bronchoalveolar lavage fluid (BALF), TGF-β, GM-CSF concentration. deoxyribose nucleotide with terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) for detection of apoptosis in lung tissue. The results compared with the asthmatic group, spleen GM-CSF in asthmatic group was significantly higher (P lt;0.01), compared with the corresponding disease, the two GM-CSF concentration in the acupuncture group were significantly lower (P lt;0.01), compared with the asthmatic group, asthma TGF acupuncture group -β concentration was significantly higher (P lt;0.05). and the asthma group, the spleen asthma eosinophils (EOS) counts were significantly higher (P lt;0.01), compared with the corresponding disease, the two acupuncture groups EOS apoptosis rate was significantly higher (P lt;0.01). Conclusion spleen increased airway inflammation inflammatory cell apoptosis by affecting the concentration of regulatory factors to achieve. acupuncturing in BALF of rats with a low level for TGF- β concentration, inhibition of GM-CSF concentration increased role. [Keywords:] Spleen asthma, cytokines, acupuncture, full three years, rat Abstract: Objective To explore the changes of TGF-β and GM-CSF in rats with experimental bronchial asthma of spleen-deficency and the regulatory