Adaptor Protein 2 Knockout on AT1 receptor-mediated endocytosis of Ang.docVIP

 PAGE \* MERGEFORMAT 13 Adaptor Protein 2 Knockout on AT1 receptor-mediated endocytosis of Ang Of: Yong Kang Jinsong Liu Quan Lou Dongmei Abstract Objective RNAi silencing the expression of AP2 2 subunit to observe whether the effect of Ang AT1R-mediated endocytosis. Methods Design and Construction of the AP2 2 RNAi expression vector and transfected into H9C2 cells were verified by Western blot silencing effect; laser confocal microscope AT1R gene silencing AP2 2-mediated endocytosis of Ang . Results Detection of siRNA recombinant protein on the expression of AP2 2, showing pSH1Si AP2 plasmid 1 plasmid can inhibit the AP2 2 protein. Ang given at different time points, we can see in 15 min, swallowed by the body to the largest number of cellular. Recombinant sub-unit of AP2 2 silence after the exogenous Ang added, may inhibit Ang AT1R-mediated endocytosis. Conclusion AT1R endocytosis mediated by the occurrence of Ang , and, like other GPCR, mainly through clathrin-dependent endocytosis manner, and the need for proteins involved in AP2. Keywords link protein 2; endocytosis; angiotensin ; AT1R Receptor-mediated endocytosis (Receptor mediated endocytosis) is the uptake of organisms recognized the main way of extracellular macromolecules. In recent years, found that G protein-coupled receptors (GPCRs) endocytic regulation of receptor function is also very important. Angiotensin (Ang ) and its GPCRs angiotensin 1 receptor (AT1R) has been considered to mediate the cardiovascular and metabolic diseases 1 end organ damage, both in the cell membrane by binding swallowed by the body in the form into the cell. In recent years, to explore the renin-angiotensin system (RAS) on the target organs and tissue pathophysiological role of intracellular signal transduction mechanism that Ang and AT1R activation in addition to the cell membrane with downstream signal transduction, the two also may be mediated through the AT1R endocytosis into the cells cont