Adrenomedullin and endothelin in renal tissue of diabetic rats.docVIP

 PAGE \* MERGEFORMAT 10 Adrenomedullin and endothelin in renal tissue of diabetic rats Analysis: Summit Hui Jun Cao Yanping Wang Xiaomei Zhao Yufeng Duan [Abstract] Objective To investigate adrenomedullin (ADM), endothelin -1 (ET-1) and its receptor in diabetic nephropathy (DN) in the pathogenesis of the dynamic changes. Methods unilateral nephrectomy in rats and streptozotocin streptozotocin (STZ) induced experimental diabetes model, rats were randomly divided into control group (A group) and diabetic group (B group), respectively, after injection of STZ drawn 2,4 and 6 weeks. detected by immunohistochemistry ADM, ET-1 and its receptor, Western blot analysis of adrenomedullin receptor (ADMR) and endothelin receptor A (EDNR-A) protein expression, RT-PCR detection of ADM and ET-1 mRNA’s expression. Results A group B group than in the expression of ADM first increased then decreased, ADMR, ET-1 and expression of EDNR-A. Conclusion ADM and ET-1 and its receptor and other vasoactive substances in the pathogenesis of DN play an important effect. [Keywords:] diabetic nephropathy; adrenomedullin; adrenomedullin receptor; Endothelin -1; endothelin receptor A Diabetic nephropathy (DN) is a serious complication of diabetes, in which the hemodynamic changes play an important role. Adrenomedullin (ADM) significantly relaxing blood vessels and lower blood pressure, natriuretic diuretic, inhibit cell growth proliferation other functions, in regulating renal function plays a significant protective effect. endothelin (ET) is a strong vasoconstrictor effect and promoting cell growth and proliferation of peptides, one of the most important ET-1. They are important vasoactive substances, more than in the past to study the changes in the blood in the renal tissue of little, through research and ET-1 ADM and its receptor expression in diabetic kidney, to further explore in vasoactive substances in the DN mechanism of action. 1 Materials and methods 1.1 Animal mode