Adult adipose derived Flk 1 mesenchymal stem cells for treatment of Duchenne muscular dystrophy Feasibility Study.docVIP

 PAGE \* MERGEFORMAT 14 Adult adipose derived Flk 1 mesenchymal stem cells for treatment of Duchenne muscular dystrophy Feasibility Study Author: Yan-Xi Sun Chao HAN Zhao Chunhua Liu Yanning 【Abstract】 In order to explore the adult adipose derived mesenchymal stem cells (adipose derived mesenchymal stem cell, AD  MSC) transplantation for treatment of Duchenne muscular dystrophy (Duchenne muscular dystrophy, DMD) the feasibility, from the fat tissue of adult mice with GFP were isolated mesenchymal stem cells (mesenchymal stem cell, MSC), using flow cytometry analysis of their cell phenotype and cell cycle; in vitro respectively, into the muscle and into the induction system and induce endothelial directional AD  MSC differentiation by immunofluorescence staining and RT  PCR for identification; via the tail vein graft AD  MSC to the CTX muscle injury model mice and mdx mice (DMD animal model) in vivo, by RT  PCR and immunofluorescence staining for the distribution of donor cells and the differentiation and conduct statistical analysis. The results showed that: from GFP mice were isolated from adipose tissue of Flk  1 MSC in vitro to differentiate into muscle cells and endothelial cells; cell transplantation, may damage the muscles in the area to detect GFP-positive muscle fibers, vascular endothelial cells and muscle stem cells; mdx mice after transplantation of anti-dystrophin on the sarcolemma (dystrophin) expression of partial recovery, skeletal muscle central core significantly reduced the proportion of muscle fibers. Conclusion: AD  MSC is a more ideal for stem cell transplantation for treatment of DMD, it is not only directed homing to the injury of muscle tissue involved in muscle fiber reconstruction, but also to improve the blood supply to alleviate the symptoms of DMD, while part of the donor cells were stem cells exists in the form, you can replace the original function of muscle stem cells to continuously repair damage to muscle tissu