Adult adipose-derived stem cells induced to differentiate into cartilage cells in vitro.docVIP

 PAGE \* MERGEFORMAT 16 Adult adipose-derived stem cells induced to differentiate into cartilage cells in vitro Author: Liang Du, Fan Yue light, Wang Haibin Lu Feng 【Abstract】 【Objective】 induced adipose tissue-derived stem cells (ADSCs) directed to differentiate into cartilage cells and were identified for cartilage tissue engineering in preparation for a large number of seed cells. 【Methods】 liposuction surgery in patients with the lipid parts of the isolation, culture and passaged, using tetrazolium compound (MTT) Determination of cell viability and cell proliferation curves depicted by flow cytometry cell lines cultured stem cells identified, the first four generations of ADSCs to induce directed differentiation of cartilage cells in parallel alcian blue staining identification; immunohistochemical detection of the direction into the cartilage after the induction of the ADSCs Ⅱ collagen expression. 【Results】 ADSCs in vitro faster and maintain a stable doubling rate of up to 13 ~ 15 generations; flow cytometry analysis showed that the cells have stem cell characteristics; through the directed differentiation of technology, ADSCs may apply to the cartilage cell differentiation, alcian blue staining and type Ⅱ collagen were positive, indicating the cells after directed differentiation of chondrocytes with normal function. 【Conclusion】 successfully induce ADSCs to the directional differentiation of chondrocytes for cartilage tissue engineering provides a reliable seed cells. 【Key Words】 adipose tissue-derived stem cells; cartilage cells / pathology; tissue engineering; cell culture The lack of cartilage-derived cartilage tissue engineering applications that hinders a major problem, because cartilage cells derived complex and difficult one to obtain sufficient number of cells in order to meet the needs of tissue engineering, so cartilage cells to explore other sources of great significance. Stem cells with multilineage differentiation p