After balloon injury of rat carotid artery smooth muscle cells the expression of mTORP70S6K.docVIP

 PAGE \* MERGEFORMAT 14 After balloon injury of rat carotid artery smooth muscle cells the expression of mTORP70S6K On: Sun Shougang Xuguang Li Wang Qiongying white political Zhu Feng [Abstract] Objective To investigate the effect of curcumin on mammalian target of rapamycin (mTOR) and ribosomal protein S6 kinase (P70S6K) in vascular smooth muscle cell proliferation. Methods 54 male SD rats were randomly divided into control, injury and drug intervention groups. carotid artery HE staining and pathological image analysis; PCNA immunohistochemistry and P70S6K observed pathological changes in tissue; real-time quantitative PCR detection of mRNA mTOR P70S6K and PCNA expression; ELISA serum interleukin 18 (IL 18) levels. Results one rat died in surgery, found during the occlusion derived 5 (respectively, 3 d drug intervention group and 7 d injury group 1, 14 and 28 d of drug intervention in group 2 ). HE staining 14 d drug intervention group compared with the injury group, the thickness of the intima and the proliferation was significantly reduced in the membrane, tissue image analysis of endometrial thickness between the three groups showed no statistically significant difference in the thickness was statistically different ( P = 0.000); immunohistochemistry results are shown injury group and the control group and intervention group differ (P = 0.045); real-time quantitative PCR mTOR P70S6K and PCNA in the mRNA injury compared with the control group was statistically significant (P lt;0.05); ELISA, compared with the control group showed curcumin can inhibit the first day of drug intervention 3,7,14 Serum IL 18 levels, with statistical significance (P all lt;0.05). curcumin can inhibit the IL 18, mTOR P70S6k and PCNA in the injured vascular smooth muscle cells and can inhibit smooth muscle cell proliferation, may become new drugs to prevent restenosis. [Keywords:] curcumin; vascular smooth muscle cells; IL-18; mTOR; ribosomal protein kinase S6 In recent