After isoflurane on acute lung injury in cecal ligation and puncture protection.docVIP

 PAGE \* MERGEFORMAT 16 After isoflurane on acute lung injury in cecal ligation and puncture protection Of: Xu Wenli 1, He Jun 1, Yin-ming 1, Pang Qingfeng [Abstract] Objective To observe isoflurane (isoflurane, ISO) on post-cecal ligation and puncture (cecal ligation and puncture, CLP) in rats after acute lung injury (acute lung injury, ALI) in rats. Methods 32 SD male rats were randomly divided into 4 groups (each n = 8): normal group (N group), received no treatment; sham operation (Sham group), underwent open abdominal surgery related; cecal ligation and puncture group (CLP group), the line CLP rat ALI model; isoflurane (ISO group), 4 h after the establishment of CLP model of intraperitoneal injection of liquid isoflurane 2 ml / kg. 12 h after abdominal aorta in each group were sacrificed, right lung to biopsy and lung wet / dry ratio (W / D) measured tissue measuring superoxide dismutase (SOD), malondialdehyde (MDA) and myeloperoxidase (MPO) content; left pulmonary bronchial lavage supernatant measuring total protein, tumor necrosis factor @ (TNF-@) and interleukin 10 (IL-10) of the content. The results showed that the lung histopathological lung CLP rats severe tissue damage, ISO less lung tissue damage. with the N group, CLP group and the ISO group, SOD decreased (P lt;0.01), the other test targets were increased (P lt;0.01). with the CLP group, ISO group SOD and IL-10 were higher (P lt;0.01); the other test were lower than CLP group (P lt;0.05 or P lt;0.01). Conclusions In this study, rats injected liquid ISO can play anti-inflammatory, antioxidant effect on ALI induced by CLP, some protective effect. [Keywords:] Acute lung injury; lung protection; isoflurane; post-processing; cecal ligation and puncture Abstract: Objective To investigate the protective effects of isoflurane (ISO) post-treatment on acute lung injury (ALI) induced by cecal ligation and puncture (CLP) in rats. Methods 32 male Sprague-Dawley (SD) rats were randomly divided into