After oral administration of ferulic acid Dangguibuxuetang tissue distribution in rats.docVIP

 PAGE \* MERGEFORMAT 8 After oral administration of ferulic acid Dangguibuxuetang tissue distribution in rats Author: Wang Yonglu, Wang Yao, Guo-Guang, Zhang Dongxu, WEI Ping [Abstract] Objective To study the rats were given ig after Dangguibuxuetang ferulic acid in the body tissue distribution properties. Methods using high performance liquid chromatography; column of Kromasil C18 (4.6 mm * 150 mm, 5 μ m); mobile phase of methanol-acetonitrile -0.2% 0.2% phosphoric acid 0.2% triethylamine aqueous solution (eighty-two past six p.m.); detection wavelength of 322 nm; column temperature 40 ℃ ; flow rate of 0.8 ml / min. The results of various organizations of ferulic acid in the 10 ~ 400 ng peak area within the range of a good linear relationship; The average recovery was 87.97% ± 1.95%; day and day of the RSD were 1.92% ~ 3.87% and 3.21% between ~ 6.39%. Ferulic acid concentration in various tissues and organs quite different. Conclusion rats after gavage for Dangguibuxuetang ferulic acid mainly in the liver and kidney distribution of ferulic acid can not be blood-brain barrier. [Keywords:] Dangguibuxuetang; ferulic acid; RP - high performance liquid chromatography; tissue distribution; rat Abstract: ObjectiveTo investigate the tissue distribution of ferulic acid in rats after oral administration of Tangkuei blood-supplementing decoction.MethodsHPLC was used; Column: Kromasil C18 (150mm * 4.6mm, 5μ m); mobile phase: 0.2% methanol acetonitrile-0.2% phosphoric acid 0.2% triethylamine solution (18:82); detection wavelength was at 322nm; temperature was at 40 ℃ ; flow velocity was 0.8ml/min.ResultsThe linear ranges of ferulic acid in the heart, liver, spleen, lung and kidney were 10 ~ 400ng. The recovery of ferulic acid was 87.97% ± 1.95% on average. The intra-day and inter-day RSD were 1.92% ~ 3.87% and 3.21% ~ 6.39%, respectively. The concentrations of ferulic acid were very different in organs. ConclusionThe major distribution organs of ferulic acid in rats