Amplification of human hematopoietic stem cell research.docVIP

 PAGE \* MERGEFORMAT 18 Amplification of human hematopoietic stem cell research Keywords:: human hematopoietic stem cells progress on Study of Techniques for Expansion of Human Hematopoietic Stem Cells Hematopoietic stem cells (llematopoietic stem cells, HSC) transplantation may cause inherited blood diseases, immune deficiency diseases and malignant tumors such as completely cured, due to lack of HSC transplantation of the following restrictions: ⑴ In order to obtain an adequate amount transplant HSC, the need for large-scale bone marrow aspiration or peripheral blood meal separation; ⑵ HSC obtained in very limited quantities; ⑶ HSC infusion of mature cells generated from the biological power poor, within 1-3 weeks after transplantation there is no direct therapy. Cultured HSC expansion before transplantation, to resolve these difficult issues. At present there are mainly two: ⑴ support screening of cytokines in vitro expansion of CD34 cells; ⑵ matrix supported by perfusion culture. Here’s the development of HSC culture, HSC and other amplification methods, and prospects are reviewed. 1 Early HSC culture and enlightenment A semi-solid culture system in 1966, Bradley et al [1] introduced a new kind of semi-solid culture method (ie, colony-forming method) for the bone marrow. This method is the use of colloidal gels to support child care, there is no cell micro-environment, only the maintenance of hematopoietic progenitor cells in 1-2 weeks, colony-forming cells can not support the growth or differentiation of HSC. Even if the can not join the nutrients, growth factors and so on, incubation time can only be extended to 2-4 weeks. By the semi-solid culture system can be inferred that the period of the early primitive HSC can not be in such a culture system in the survival, proliferation. 2Dexter culture system Dexter et al [2] in 1977 established the bone marrow liquid culture system. This culture method is based on the establishment of a support lay