Amphetamine on the mouse interleukin-10 release of dopamine D3 receptors and the regulation of.docVIP

 PAGE \* MERGEFORMAT 17 Amphetamine on the mouse interleukin-10 release of dopamine D3 receptors and the regulation of Of: Chen Yanjiong, Zhujun Yan, Zhang Hongbo, Wei Shuguang, Yanchun Xia, Zheng Haibo, Ren Huixun, Chen Teng [Abstract] Objective amphetamine (AM) on lipopolysaccharide (LPS) stimulated murine interleukin-10 (interleukin 10, IL 10) and the release of dopamine D3 receptors in the impact of the process involved in the regulation of . Methods The experiment : use of dopamine D3 receptor knockout mice (D3RKO) and similar genetic background of wild-type mice (C57BL/6J), intraperitoneal injection of AM (5mg/kg) 10min after the injection of LPS (150g / kg) stimulation, different time points after LPS administration (0,30,60,90,120,240 min) to IL 10 cardiac puncture for blood tests to observe the different time points D3RKO and C57BL/6J mice the secretion of IL 10 situation. Experiment : in experimental mice as determined by the secretion of IL 10 highest points in time to observe the different doses of AM (0,2,5,10 mg / kg) and C57BL/6J mice on D3RKO secretion of IL 10. Results Experiment : AM 5mg/kg LPS injection in D3RKO and C57BL/6J mice can cause increased secretion of IL 10, which reached the maximum 60min, compared with the saline control group significantly (P lt;0.05). Experiment : C57BL/6J mice were the role of AM 5mg/kg after the most significant increase in IL 10, D3RKO effects in mice after the AM 2mg/kg increased IL 10 most significant difference compared with the saline control group were statistically significant ( P lt;0.05), and there are also significant differences between the two. Conclusion AM suppression of immune function may be through increased secretion of IL 10 works, a small dose of AM in mice can cause D3RKO blood IL 10 high, suggesting that dopamine D3 receptors may be involved in the AM inhibitory effect on the regulation of immune function. [Keywords:] amphetamine understand interleukin 10, lipopolysaccharide d