Angiopoietin -1 in diabetic rat retinal microvascular leakage protection.docVIP

 PAGE \* MERGEFORMAT 16 Angiopoietin -1 in diabetic rat retinal microvascular leakage protection [Abstract] Objective angiopoietin -1 (Ang-1) on early retinal microvascular leakage in diabetic rats, and vascular cell apoptosis and to explore its mechanism. Methods of streptozotocin (STZ) prepared SD diabetic rats. were randomly divided into 6 groups: diabetes March group (DM3), 6 month group (DM6), Ang-1 treatment for 3 month group (ANG3), 6 month group (ANG6) and normal controls in March group (CON3), 6 month group (CON6). ANG rats after injection of each eye ball Ang-1 solution 0.05 mL/15 d (4 g / mL). after the expiration of the rats, intravenous injection of different thiocyanate glucan, preparation of retinal blood vessels stretched; quantitative immunohistochemical expression of retinal VEGF; Western blot assay for quantification of the expression of Survivin retina. application of the data for statistical analysis package SPSS. Results DM3 group of large rat retinal capillary distorted, irregular, showing no perfusion, ANG3 group showed significant improvement. DM6 group end capillaries to a “Bacillus” kind of proliferation, ANG6 group did not find. retinal expression of VEGF were significantly higher DM , compared with the CON group difference was significant (P lt;0.01), ANG group compared with DM group decreased expression, the difference was statistically significant (P lt;0.05). rat retina the expression of Survivin protein was no CON, ANG3 group expression higher than DM3 highly significant difference (P lt;0.01), DM6 and ANG6 difference between groups was not significant (Pgt; 0.05). Conclusions Ang-1 inhibited the leakage of retinal capillaries in diabetic rats and diabetic rats inhibited the increase of retinal VEGF expression; can prevent apoptosis of capillary cells in diabetic rat retina. [Keywords:] diabetic retinopathy; microvascular leakage; angiopoietin -1; apoptosis Abstract: Objective The paper aims to explore the effect of Ang