Application of VEGF after severe trauma prevention and control limbs experimental study of osteoporosis.docVIP

 PAGE \* MERGEFORMAT 10 Application of VEGF after severe trauma prevention and control limbs experimental study of osteoporosis [Abstract] Objective To study the local application of vascular endothelial growth factor (vasular endothelial growth foutor, VEGF) serious physical injury prevention and treatment of osteoporosis after local, to provide experimental basis for clinical application. Methods According to Allen’s vertical combat weight (weight dropping, WD method) New Zealand rabbits produced severe distal radial injury animal model. The experimental animals were randomly divided into experimental group (injection pHVEGF165 plasmid) and the control group (topical application of saline group), 15 rats in each group. experimental group injured immediately after the local (distal radius around) injection pHVEGF165 plasmid 50 ug, saline injection control group. 2,4,6,8 weeks of observation after the administration of blood flow and distal radius bone mineral density. were killed 8 weeks after treatment animals for pathological examination. Results The 2,4,6,8 weeks after injury, blood flow than the control group, the distal radius bone mineral density was significantly higher (P lt;0.05). experimental pathology the bone increased trabecular thickening. Conclusion plasmid injection pHVEGF165 after severe trauma can improve limb blood flow and prevent the occurrence of localized osteoporosis. [Keywords:] serious physical injury, blood flow, bone density, osteoporosis Abstract: Objective To approach the effect of local application of VEGF for curing bone rarefaction after serious limbs crush injury, and to provide experimental basis for clinical treatment.Methods Animal model of New Zealand rabbit’s limbs (distal radius) crush injury were produced with reference to Allen’s vertical combat weight (weight-dropping, WD method) and were randomly divided into experimental group (local injection with pHVEGF plasmid) and the control group (local injecting with