Around the sciatic nerve CCI rats clonidine on proinflammatory cytokine expression.docVIP

 PAGE \* MERGEFORMAT 16 Around the sciatic nerve CCI rats clonidine on proinflammatory cytokine expression [Abstract] Objective: To observe the damage around the injection of clonidine on the sciatic nerve sciatic nerve ligation model of chronic (chronic constriction injury, CCI) in rats the expression of proinflammatory cytokines, and to explore @ 2 - adrenergic receptor agonists are already formed produce neuropathic pain. METHODS: 32 male SD rats were randomly divided into 4 groups of 8. Sham group: only the separation of the skin, muscle, no ligation of sciatic nerve; Sal groups: saline group, after the formation of CCI; Clo group: after the formation of CCI clonidine group; Clo + Yo Group: After the formation of CCI and yohimbine clonidine group. 3 d after the acute injection animals were killed ipsilateral spinal cord L4, L5, L6 dorsal root ganglia Day to automated chemiluminescence assay TNF-@ and IL-6 concentration. Results: After 7 d starting from the CCI rat dorsal root ganglia TNF-@ and IL-6 concentration was significantly higher. sciatic nerve injury around the injection of coke CCI will be able to inhibit the cause of the spinal dorsal root ganglia TNF-@ and IL-6 expression (P lt;0.01). Conclusion: The sciatic nerve injury around the injection of clonidine can reduce the expression of proinflammatory cytokines. [Keywords:] clonidine; peripheral injection; CCI rats; neuropathic pain; TNF-@; IL-6 Abstract Objictive: To assess the effect of perineural clonidine on the expression of TNF-@ and IL-6 in the rats with chronic constriction injury (CCI) in the sciatic nerve. Methods: Thirty-two male SD rats were randomly divided into four groups (18 for each): sham group, Sal group (CCI + saline), Clo group (CCI + clonidine) and Clo + Yo group (CCI + clonidine + yohimbin). The rats were killed 3 days after the injection of medicine mentioned above and spinal cord DRG of L4, L5, L6 were taken for determining the concentration of TNF-@ and IL-6 of DRG