Array of interdigital cell chip dielectrophoresis enrichment process.docVIP

 PAGE \* MERGEFORMAT 16 Array of interdigital cell chip dielectrophoresis enrichment process Of: Haodun Ling Xu Yi Wen Zhi Qiang Cao Yu was the snow [Abstract] interdigitated electrode array dielectrophoresis (Dielectrophoresis, DEP) chip, integrated building control systems analysis and DEP chip, application Coventorware finite element analysis software simulation of the chip surface, the electric field distribution; to red blood cells and colon cancer cells sample is analyzed, and realized in the two cell samples on the chip positioning the positive and negative dielectrophoresis enrichment. It was found that the AC signal amplitude Vp p is to determine the main cause of the efficiency of DEP concentration, communication signal frequency f and the buffer solution is dielectrophoresis to change the type of cell parameters; in 0.9% NaCl, the applied rate of 10 and 3 MHz, 5 V AC voltage frequency of colon cancer cells positive dielectrophoresis (Positive dielectrophoresis, pDEP) and negative dielectrophoresis ( Nagetive dielectrophoresis, nDEP) enrichment efficiency was 87.2% and 84.8%. [Keywords:] chip dielectrophoresis, cell enrichment, interdigitated electrode array 1 Introduction Dielectrophoresis chip (Microchip dielectrophoresis) is based on dielectrophoresis separation principle and MEMS processing technology based on new analytical techniques, the biochemical analysis of samples was superior to conventional detection methods. In recent years, chip dielectrophoresis from the initial the simple accumulation of neutral particles operations, extended to the bacteria, cells, viruses, nano materials and substances such as metal particles enrichment, separation and control, etc. [1 ~ 4]. dielectrophoresis (Dielectrophoresis, DEP) and fluid forces [5], electric field strength [6], laser tweezers [7] study mode coupling have been reported, while dielectrophoresis chip technology coupled with other analytical methods is a new research direction. die