Arsenic trioxide on human gastric cancer xenografts in nude angiopoietin2 and vascular endothelial growth factor expression.docVIP

 PAGE \* MERGEFORMAT 18 Arsenic trioxide on human gastric cancer xenografts in nude angiopoietin2 and vascular endothelial growth factor expression Of: Xiao Yan-Feng, Liu Shaanxi, Wu De, Chen Xi, Ren Lifen [Abstract] Objective To study the arsenic trioxide (As2O3) on tumor angiopoietin 2 (Ang 2) and vascular endothelial growth factor (VEGF) expression. SGC7901 gastric adenocarcinoma cell line method employing 30 nude mice inoculated, the establishment of solid tumor model As2O3 and then randomly divided into high dose (5mg/kg), low dose (2.5mg/kg) treatment group and control group, n = 10. As2O3 treatment group received intraperitoneal injection of 10d, the control group received normal saline. measuring tumor volume inhibition rate was calculated, and calculated Immunofluorescence CD31 vessel density by immunohistochemistry the expression of Ang 2 was determined by immunofluorescence confocal detection of the expression of VEGF. Results of arsenic trioxide treatment could inhibit the growth of tumor masses, 2.5mg/kg and 5mg/kg As2O3 treatment inhibited tumor growth rates were 30.33% and 50.85%. As2O3 treatment group, microvessel density (MVD), VEGF expression levels of fluorescence were significantly lower than the control group (P lt;0.01). Ang 2 mainly in vascular endothelial the cytoplasm of cells were strongly positive expression, can be clearly labeled blood vessels within the tumor, the control group and treatment group As2O3 Ang 2 expression in vascular endothelial cells no significant difference in strength. Conclusion As2O3 on the expression of Ang 2 had no effect, inhibition of tumor treatment in As2O3 the case of VEGF expression in cells, Ang 2 expression can promote the degradation of blood vessels. Ang 2, mainly in the expression of new endothelial cells, it may serve as markers of angiogenesis, tumor angiogenesis research into new targets. [Keywords:] Arsenic trioxide, angiopoietin 2, vascular endothelial growth factor, angiogenesis, can