Artificial joints of metal wear particles in vitro experimental study prepared by separation method.docVIP

 PAGE \* MERGEFORMAT 27 Artificial joints of metal wear particles in vitro experimental study prepared by separation method Abstract design an in vitro preparation, separation of metal wear particles of artificial joints method and verify that the particles used in medical experiments is feasible. [Methods] titanium aluminum vanadium alloy, cobalt chromium molybdenum alloys were made of ball-milling pot (national invention patent, application number: 03,142,073.7), ball-milling the tank with the same kinds of materials with the wear block; to the ball-milling the tank into the simulated biological fluids; vibration ball mill to be particle suspension. Gradient centrifugation to obtain metal particles. Pairs of particles: (1) identification of elemental composition; (2) identification of particle size and particle size analysis; (3) scanning electron microscope observation of surface morphology of the particles; (4) particles and J774A. 1 macrophages co-cultured observe the phagocytosis of particles. [Results] by the success of this method to generate and isolate a large number of about 1 μm in diameter titanium alloy and cobalt-chromium molybdenum alloy particles. (1) elemental analysis confirmed that the entire treatment process without impurity contamination; (2), titanium alloy, the average particle diameter of Dv90: 1. 009, Co-Cr-Mo particles, the average diameter of Dv90: 1. 008, particle size distribution curve into a positive state of ; (3) scanning electron microscope images showed uniform particle size, shape, mostly irregular, and the body is very similar to particles; (4) J774A. 1 macrophage phagocytosis two kinds of materials can be full of particles. [Conclusion] This method can continue to produce a large number of metal prosthesis wear particles, the particles can be produced very well in all respects to simulate in vivo wear particles for the future loosening of artificial joint prosthesis-related research in vivo, in vitro provide a re