Atomic Fluorescence Determination of Selenium in Food.docVIP

 PAGE \* MERGEFORMAT 9 Atomic Fluorescence Determination of Selenium in Food [Abstract] using atomic fluorescence spectrometry Determination of selenium, selenium relative standard deviation of 1.2%, the detection limit of 0.40mg/ml. The linear range of 0 ~ 400μ g / L, the correlation coefficient of 0.9999. Recoveries were 95.1% ~ 103.8%. [Keywords:] Selenium; atomic fluorescence; Food Selenium is an essential trace element, but the intake of too much harm to human health. In recent years, food processing arbitrary in some foods to strengthen the tendency of selenium. In order to protect human health, and therefore strengthen the selenium in the food, the water content of the monitoring efforts. Common methods of fluorescence spectrometry, atomic absorption spectrometry and colorimetry, etc. assay is accurate, but cumbersome, and the reagents DAN toxic and to be imported. Flame atomic absorption spectrometry method of low sensitivity, graphite furnace method has serious matrix interference assay is simple and low sensitivity. Atomic Fluorescence Determination of selenium to overcome the shortcomings of the above method is a simple and sensitive approach. An experimental part of the 1.1 Reagents and Instruments Shan Kwong Road, atomic fluorescence spectrophotometer (Beijing Rayleigh Analytical Instruments AF-610), equipped with a computer processing system, selenium hollow cathode lamp: Beijing Vacuum Electronics Research Institute, nitric acid (excellent grade pure), perchloric acid (U grade pure), hydrochloric acid (excellent grade pure), mixed acid: nitric acid + perchlorate (41), potassium hydroxide (AR), potassium borohydride solution (15g / L) that is about to take 2g KOH dissolved in 200ml of pure water, adding 15g of potassium borohydride dissolved and made with cotton filter, diluted with water to 1000ml, shake. The existing service is appropriate to use. Potassium ferricyanide (100g / L): Weigh 10.0g potassium ferricyanide (K3Fe (CN) 6)