Attenuated Salmonella plasmid carrying siRNASTAT3 in mice transplanted prostate cancer treatment.docVIP

 PAGE \* MERGEFORMAT 21 Attenuated Salmonella plasmid carrying siRNASTAT3 in mice transplanted prostate cancer treatment Of: Liuxi Dong Zhou Yue Zhou Shu Fang Wei Yanhong Zhou Qingwei group with Cao Jian Zhao Xue [Abstract] Objective To observe the attenuated Salmonella vector carrying siRNA STAT3 in mice transplanted prostate cancer treatment, analysis of its mechanism. Methods Construction of the mouse prostate tumor model, randomized, will be transformed with siRNA STAT3 attenuated Salmonella typhimurium by plasmid local injection to build a successful model in mice. general condition of mice were observed to record changes in tumor volume, RT PCR and Western bolt analysis STAT3 and its downstream gene Bcl 2, c Myc, HiF1, cyclinD1 the mRNA and protein expression levels , bacteria were observed distribution of plank, HE staining of tumor morphology, immunohistochemistry STAT3, HiF1 and PCNA protein expression. Results mouse prostate tumor model was successfully constructed plasmid carrying siRNA STAT3 transplantation Salmonella group body prostate cancer growth was inhibited, reducing tumor weight, ceiling assay showed attenuated Salmonella bacteria distribution of tumor characteristics and addicted to long-term survival in the tumor, intrahepatic distribution of the second, again for the lung, the distribution in the spleen minimum, pGC Si STAT3 of tumor tissue STAT3 mRNA and protein expression was significantly reduced, and its downstream gene Bcl 2, c Myc in the mRNA and HiF1, cyclinD1 protein expression levels are different degrees of inhibition, immunohistochemistry showed pGC Si STAT3 of tumor tissue STAT3, HiF1 and PCNA was decreased. Conclusion attenuated Salmonella as a transport vector plasmid carrying siRNA STAT3 downstream genes by regulating the expression of STAT3 and induce apoptosis of prostate cancer in mice transplanted with significant inhibition effect. [Keywords:] prostate cancer, attenuated Salmonella, STAT3, RNA interferenc