Bone sialoprotein expression on osteosarcoma cell invasion and metastasis characteristics of.docVIP

 PAGE \* MERGEFORMAT 15 Bone sialoprotein expression on osteosarcoma cell invasion and metastasis characteristics of Author: Huang Tao, Min Chen, Guo Jin, Zhang Yi North, Liao Hui 【Abstract】 Objective To construct the human bone sialoprotein (BSP) sense and antisense eukaryotic expression vector, to study the invasion and metastasis of osteosarcoma cell characteristics. Methods The PCR-amplified open reading frame of human BSP sequence, with the vector pIRES2  EGFP linked to constitute a recombinant antisense expression plasmid. By liposome-mediated transfection into MG  63 cells, by Western blot detection of cell protein. With reconstituted basement membrane invasion assay and scratches Experimental Determination of the invasiveness of osteosarcoma cell effects. The result successfully constructed a human BSP antisense RNA eukaryotic expression vector, after transfection effectively expressed hBSP, compared with the control group, just after transfection vectors so that MG  63 cells hBSP elevated levels of protein expression, invasiveness enhancement; antisense vector transfected to MG  63 cells hBSP reduce the level of protein expression, invasiveness is inhibited. Conclusion BSP expression changes may be the process of invasion and metastasis of osteosarcoma play an important role, BSP decreased expression can inhibit the invasive ability of osteosarcoma. Keywords: bone sialoprotein gene; gene expression; tumor metastasis; Osteosarcoma Expression of BSP Influence on Behavior of Invasion and Metastasis of Osteosarcoma Abstract: Objective To construct sense and antisense human bone sialoprotein (BSP) eukaryotic expression vector and observe influence of its expression in human osteosarcoma cell line MG  63 cells about invasion and metastasis of osteosarcoma.Methods An open reading frame of human BSP was amplified by PCR method and was reconstructed into the eukaryotic expressive vector pIRES2  EGFP to construct the hBSP se