Cisplatin-resistant ovarian cancer cell lines induced apoptosis time-and dose A2780-DDP compliance.docVIP

 PAGE \* MERGEFORMAT 23 Cisplatin-resistant ovarian cancer cell lines induced apoptosis time-and dose A2780/DDP compliance Author: Fu-chun, Li Guangyi, Liu Xiaoying, Qiu-Hua Lin, Liu Feng-ying, Xie Yong 【Abstract】 Objective To investigate the role of different concentrations of cisplatin at different time-sensitive and drug-induced apoptosis in ovarian cancer cell line variation and apoptosis pathway-related protein expression. Methods cisplatin-resistant ovarian cancer cell lines A2780/DDP and sensitive cell lines A2780 for the study. In cell culture, based on the use of MTT assay, in situ labeling method, routine immunohistochemistry and Western blot methods, the effects of different doses of cisplatin (5,10,20,40 μmol / L) and the role of different time (24 , 48,72 h) to the A2780 and A2780/DDP cell apoptosis and this dynamic process caspase  2 (Caspase  2), and  3 (Caspase  3) expression. The results of the A2780 and the cisplatin-induced apoptosis A2780/DDP cell lines in a time-and dose-compliance. Apoptosis proteins Caspase  2 and Caspase  3 activation enhances cisplatin-induced apoptosis, its expression was a right time and the cisplatin dose compliance. Conclusions (1) in cisplatin-resistant mechanism, the drug in vivo distribution and metabolism of the effective concentration of plasma and cellular rejection caused by the drug cisplatin into the cells, there are some obstacles may be the factors that reduce the sensitivity to cisplatin. (2) Caspase  2 and the delayed activation of caspase  3 protein and activity decrease are likely to affect apoptosis-resistant A2780/DDP reasons. Keywords: cisplatin-resistant; apoptosis; Caspase  2; Caspase  3; human ovarian cancer Cisplatin  Induced Apoptosis in Cisplatin  resistant Ovarian Cancer Cell Line A2780/DDP on Time  and Dose  Dependence Keywords:: Cisplatin  resistance; Apoptosis; Caspase  2; Caspase  3; Human ovarian caner