Comparison of Haemophilus influenzae Save.docVIP

 PAGE \* MERGEFORMAT 9 Comparison of Haemophilus influenzae Save Of: Liu Jinjun Wang Hong Zhang Yu Qiao Haixia Zhang Yanxia Li Caiqing properly [Keywords:] Haemophilus / flu; specimens; Save / Biology In the microbiological testing work, the success of bacterial species preservation, biological characteristics for the study of bacteria, the mechanism of resistance variation, monitoring trends of nosocomial infection and the development of pathogens and other essential drugs. I Department of influenza clinical isolates in order to Haemophilus preserved for future preparation of the monoclonal antibodies, other items of vaccine development, we tried four Haemophilus influenzae preservation methods, including: serum glycerol broth method, skim milk method, paper method and vacuum freeze-drying. Save 1 month, 3 months, 6 months and 1 year after the recovery strains are compared, trying to find the best method of preservation. 1 Materials and methods 1.1 strains and clinical specimens from a strain of the First Affiliated Hospital of Hebei North University pediatric clinic and kindergarten children in the normal pharyngeal isolates, a total of 32 strains were identified as the phenomenon by satellite Haemophilus influenzae. 1.2 Methods 1.2.1 Serum glycerol glycerol broth broth method after mixing 1:1 121.3 15min, cooled sterilized with 10% serum blending, take 0.5mL min mounted in 1mL eppendorf in closing lid placed on the bracket, but Do not Gaiyan picked chocolate plate Haemophilus influenzae vigorous growth of several colonies in this medium grinding, 4 refrigerator stable after 1h, into the -20 preservation. 1.2.2 skim milk method [1] 5 times bought fresh milk to boil, fully defatted take 0.5mL min mounted in 1mL eppendorf in closing lid placed on the bracket but not Gaiyan 115.6 15min. Picked Haemophilus influenzae number of colonies in this medium grinding, 4 refrigerator stable after 1h, into the -20 preservation. 1.2.3 disk d