Compound Naodesheng purification process of total flavonoids.docVIP

 PAGE \* MERGEFORMAT 12 Compound Naodesheng purification process of total flavonoids Of: Wang Guangzhong, Lee Bridge, Song Tian, #8203;#8203;Zhang, Fan Wenjuan, Liu Yan Wen [Abstract] Objective To study the compound Naodesheng the best in the purification process of total flavonoids. Methods orthogonal and single factor test, the washout rate of total flavonoids and content as an indicator to investigate use of D-101 resin enrichment, Compound Naodesheng purification of total flavonoids in the conditions and parameters. The results for the optimization of purification: the sample concentration 0.1 g / mL, flow rate 2 BV / h, the high ratio of 1:7 resin diameter, adsorption after the first 5 BV The water cleared, and then 5 BV 40% ethanol elution. Extract from the content of total flavonoids 80.57%, the transfer rate of 83.76% of total flavonoids. Conclusions are preferred purification process of total flavonoids content, technology is simple, suitable for industrial production. [Keywords:] D-101 resin, the compound Naodesheng, total flavonoids, purification process Abstract: Objective To confirm the optimum purification of the total flavonoids in Naodesheng. Methods According to the elution rate and the purity of the products, the preparation performance of total flavonoids by D-101 macroporous resin was investigated, and its parameters were optimized by the orthogonal test and single-factor test. Results The optimum conditions were as follows: the concentration of sample was 0.1 g / mL, the adsorptive speed of flow was 2 BV / h, the resin bed ф: L proportion was 1:7. The adsorbing column was eluted with 5 BV water, and then eluted with 5 BV 40% ethanol solution. The content of total flavonoids in the extract was 80.57%, and the yield of the total flavonoids was 83.76%. Conclusion The content of the total flavonoids obtained was high by the optimum purification conditions. The technique is simple and adaptable for production. Keywords:: D-101 mac