Cordycepin on tumor necrosis factor-induced vascular smooth muscle cell proliferation and syndecan4 protein expression.docVIP

 PAGE \* MERGEFORMAT 17 Cordycepin on tumor necrosis factor-induced vascular smooth muscle cell proliferation and syndecan4 protein expression Of: Yu, Ouyang, Yan Wen Lai, Jian Burrell 2, Xu Dingli [Abstract] Objective To observe cordycepin on tumor necrosis factor @ (TNF @) stimulation of isolated rat vascular smooth muscle cells (VSMCs) proliferation and protein expression of syndecan 4. Methods rat aortic VSMCs, respectively, the final application concentration of 20 ng / mL TNF @, 20 mol / mL cordycepin, 40 mol / mL cordycepin, 20 ng / mL TNF @ +20 mol / mL cordycepin, 20 ng / mL TNF @ +40 mol / mL cordycepin the role of 24 h, and the control group for comparison. using MTS / PMS method to determine the proliferation of VSMCs state, the use of Western blot Western blot determination of VSMCs in syndecan 4 expression. Results (1) compared with the control group, TNF @ group can significantly stimulate the proliferation of rat VSMCs (P lt;0.05), and different doses of cordycepin on rat alone had no significant effect on the proliferation of VSMCs (Pgt; 0.05). TNF @ joint action group with co-cordycepin TNF @ group comparison, VSMCs proliferation was significantly reduced (P lt;0.05). (2) Western blot Western blot tests showed that: compared with control group, TNF @ group significantly stimulated VSMCs of syndecan 4 expression (P lt;0.05), and Cordyceps Su different doses alone on VSMCs of syndecan 4 expression had no significant effect (Pgt; 0.05). TNF @ cordycepin common joint action group with TNF @ group, VSMCs of syndecan 4 expression was significantly decreased (P lt;0.05) . Conclusion cordycepin could inhibit TNF @ induced VSMCs proliferation and cell syndecan 4 expression. [Keywords:] syndecan 4; cordycepin; tumor necrosis factor @; vascular smooth muscle cells; cell proliferation Abstract: Objective To investigate the effects of cordycepin on the proliferation of rat vascular smooth muscle cells (VSMCs) and expression of syndecan 4 protei